Novo Nordisk A/s

Other

Lever Brothers Company, Division of Conopco, Inc.

Unilever Plc

Novozymes A/s

Lever Brothers Company

Johan Haverkamp

Ole Hvilsted Olsen

Sven Hastrup

Sven Branner

Maarten Robert Egmond

Eric Casteleijn

Dorrit A Aaslyng

Merete Simonsen

Arnoldus Theodorus Anthonius Mooren

John David Marugg

Wouter Musters

Poul Lindegaard

Nina Eriksen

Cornelis G Van Kralingen

Peter J Hall

Michael Schmidt

Jakob De Vlieg

Leif N&oslash;rskov-Lauritsen

Ton Swarthoff

John D Marugg

Leif No slashedrskov-Lauritsen

Leif Norskov-Lauritsen

Arnoldus Theodorus Mooren

Eric Castelijn

Hendrik E De Jong

The present invention relates to enzymes produced by mutating the genes for a number of subtilisin proteases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved wash performance in comparison to their wild type parent enzymes. The enzymes are well-suited for use in detergent compositions.

Modified subtilisins and detergent, compositions containing same

Modified subtilisins and detergent compositions containing same

The present invention relates to modified subtilisins comprising a mutation in an amino acid sequence of a subtilisin at a position selected from the group consisting of 1, 2, 3, 4, 6, 9, 10, 12, 14, 15, 17, 18, 19, 20, 21, 22, 24, 25, 27, 36, 37, 38, 40, 41, 43, 44, 45, 46, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 75, 76, 77, 78, 79, 87, 89, 91, 94, 97, 98, 99, 100, 101, 103, 104, 105, 106, 107, 108, 109, 112, 113, 115, 116, 117, 118, 120, 126, 128, 129, 130, 131, 133, 134, 136, 137, 140, 141, 143, 144, 145, 146, 155, 156, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 170, 171, 172, 173, 181, 182, 183, 184, 185, 186, 188, 189, 191, 192, 194, 195, 197, 204, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 259, 260, 261, 262, 263, 265, 269, 271, 272 and 275. The present invention also relates to detergent compositions comprising a modified subtilisin of the present invention.

This invention relates to enzymes, to rDNA techniques applicable for example to their production, to mutated genes, vectors and mutant and transformed microorganisms useful in their production, and to their uses including for example enzymatic detergent and cleaning compositions containing them.

Detergent compositions comprising a modified subtilisin

A detergent composition contains a combination of two different surfactants, one micellar phase and one lamellar phase, at least one of the surfactants being a glycolipid biosurfactant. Preferred micellar phase biosurfactants are rhamnolipids, sophoroselipids and cellobioselipids, advantageously used in combination with non-glycolipid anionic or nonionic surfactants; while preferred lameilar biosurfactants are trehaloselipids, glucoselipids, and rhamnolipids, advantageously used in combination with micellar biosurfactants. The detergent compositions show enhanced oily soil detergency in fabric washing even when the glycolipid biosurfactants used individually are poor detergents.

Laundry detergent composition containing synergistic combination of

Subtilisin mutants

A detergent composition contains a combination of two different surfactants, one micellar phase and one lamellar phase, at least one of the surfactants being a glycolipid biosurfactant. Preferred micellar phase biosurfactants are rhamnolipids, sophoroselipids and cellobioselipids, advantageously used in combination with non-glycolipid anionic or nonionic surfactants; while preferred lamellar biosurfactants are trehaloselipids, glucoselipids, and rhamnolipids, advantageously used in combination with micelar biosurfactants. The detergent compositions show enhanced oily soil detergency in fabric washing even when the glycolipid biosurfactants used individually are poor detergents.

Synergistic dual-surfactant detergent composition containing

The inclusion of proteases with an isoelectric point of less than 10.0 in a detergent composition which comprises a certain, immunologically defined, class of lipases causes a significantly reduced effect of proteases on lipases in comparison with other proteases. The overall performance of the lipase-containing detergent compositions is substantially less affected by these proteases with a pI of less than 10.0.

Enzymatic detergent composition

Growing community of inventors

Bergschenhoek, Netherlands

Johan Haverkamp