Astrego Diagnostics Ab

Johan Elf

Özden Baltekin

Ove Öhman

Martin Lovmar

Mikael Olsson

Dan I Andersson

George M Church

Michael Read

Elias Amselem

Petter Hammar

Ove Ohman

Ozden Baltekin

ÖZden Baltekin

A microfluidic device () comprises a substrate () having spatially defined and separated cell compartments () configured to accommodate cells. A respective first end () of the spatially defined and separated cell compartments () is in fluid connection with a flow input channel (). The microfluidic device () comprises at least one identification surface () comprising immobilized affinity molecules configured to capture cells of a species or serotype or of a group of species or serotypes.

Microfluidic device for cell characterization

A capturing of target cells from a biological sample is achieved by inducing a flow of the biological sample in a flow channel () of an upstream microfluidic device (). Target cells present in the biological sample are captured in cell channels () of the upstream microfluidic device (). Once at least a minimum number of target cells are captured in the cell channels (), the flow of the biological sample in the flow channel is reduced and are verse flow is applied at the upstream microfluidic device () to release the target cells captured in the cell channels () of the upstream microfluidic device () and enable transfer the target cells into cell channels () of a downstream microfluidic device ().

Cell capture in microfluidic devices

Phenotyping of cells involves loading a biological sample into a microfluidic device () comprising cell compartments () to capture biological material, including target cells, in the sample in the cell compartments (). A subset (A) of the cell compartments () is identified as comprising target cells exhibiting target phenotype characteristic(s) as determined based on monitoring biological material in the cell compartments () prior to addition of a test agent. The biological material is exposed to a test agent and a phenotypic response of the target cells to the test agent is determined based on monitoring target cells in the identified subset (A) of the cell compartments (). The phenotyping of the target cells is thereby not overshadowed by the response of other cells and non-cell material present in the biological sample.

Phenotypic characterization of cells

A microfluidic device () comprises a substrate () having a flow input channel () in fluid connection with a first fluid port () and a flow output channel () in fluid connection with a third fluid port () and cell channels () disposed between the flow input channel () and the flow output channel (). The cell channels () comprise a respective obstruction () designed to prevent the target cells from passing the respective obstruction () and into the flow output channel (). The microfluidic device () also comprises a pre-filter () with a filter channel () in fluid connection with a first filter port () and pre-filter channels () adapted to accommodate the target cells. A respective first end () of the pre-filter channels () is in fluid connection with the filter channel () and a respective second end () of the pre-filter channels () is in fluid connection with the flow input channel ().

Microfluidic device

A microfluidic device comprises a substrate transparent for imaging and having a plurality of spatially defined and separated cell channels having a dimension to accommodate cells in monolayer. A respective first end of the cell channels is in fluid connection with a flow input channel having a first end in fluid connection with a first fluid port and a second end in fluid connection with a second fluid port. A respective second end of the cell channels is in fluid connection with a first end of a respective wash channel having a second end in fluid connection with a flow output channel. The flow output channel is in fluid connection with a third fluid port. The wash channels have a dimension too small to accommodate the cells.

A library of cell strains is characterized by culturing cells at spatially defined and separated positions in a culturing device () and determining a phenotypic characteristic of each cell strain in the culturing device (). The cells are fixated at the spatially defined and separated positions in the culturing device () followed by in situgenotyping a respective variable region () of each cell strain at the spatially defined and separated positions in the culturing device (). Each respective phenotypic characteristic is connected to each respective genotype based on the spatially defined and separated positions in the culturing device ().

Phenotypic characterization and in situ genotyping of a library of genetically different cells

A microfluidic device () comprises a substrate () transparent for imaging and having a plurality of spatially defined and separated cell channels () having a dimension to accommodate cells in monolayer. A respective first end () of the cell channels() is in fluid connection with a flow input channel () having a first end () in fluid connection with a first fluid port () and a second end () in fluid connection with a second fluid port (). A respective second end () of the cell channels () is in fluid connection with a first end () of a respective wash channel () having a second end () in fluid connection with a flow output channel (). The flow output channel () is in fluid connection with a third fluid port (). The wash channels () have a dimension too small to accommodate the cells.

Growing community of inventors

Uppsala, Sweden

Johan Elf