Natera, Inc.

Johan Baner

Matthew Rabinowitz

Zachary Demko

George Gemelos

Matthew Micah Hill

Milena Banjevic

Allison Ryan

Bernhard Zimmermann

Styrmir Sigurjonsson

Matthew Hill

Bernhard Zimmerman

Huseyin Eser Kirkizlar

Onur Sakarya

Joshua Babiarz

Tudor Pompiliu Constantin

Lane A Eubank

Carl Oscar Fredrik Dahl

Olof John Ericsson

The present disclosure provides methods for determining the ploidy status of a chromosome in a gestating fetus from genotypic data measured from a mixed sample of DNA comprising DNA from both the mother of the fetus and from the fetus, and optionally from genotypic data from the mother and father. The ploidy state is determined by using a joint distribution model to create a plurality of expected allele distributions for different possible fetal ploidy states given the parental genotypic data, and comparing the expected allelic distributions to the pattern of measured allelic distributions measured in the mixed sample, and choosing the ploidy state whose expected allelic distribution pattern most closely matches the observed allelic distribution pattern. The mixed sample of DNA may be preferentially enriched at a plurality of polymorphic loci in a way that minimizes the allelic bias, for example using massively multiplexed targeted PCR.

Methods for non-invasive prenatal ploidy calling

The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

Methods for simultaneous amplification of target loci

Methods for preparing a biological sample obtained from an individual for use in a genetic testing assay

Methods for non-invasive prenatal paternity testing are disclosed herein. The method uses genetic measurements made on plasma taken from a pregnant mother, along with genetic measurements of the alleged father, and genetic measurements of the mother, to determine whether or not the alleged father is the biological father of the fetus. This is accomplished by way of an informatics based method that can compare the genetic fingerprint of the fetal DNA found in maternal plasma to the genetic fingerprint of the alleged father.

Methods for amplification of cell-free DNA using ligated adaptors and universal and inner target-specific primers for multiplexed nested PCR

Methods for preparing a DNA fraction from a biological sample for analyzing genotypes of cell-free DNA

Methods for non-invasive prenatal paternity testing

Methods for nested PCR amplification of cell-free DNA

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San Francisco, CA, United States of America

Johan Baner