Danmarks Tekniske Universitet

Københavns Universitet

University of Copenhagen

Evolva Sa

Chr. Hansen Natural Colors A/s

Danmarks Teniske Univesitet

Technical University of Denmark

Johan Andersen-Ranberg

Birger Lindberg Møller

Finn Thyge Okkels

Bjørn Madsen

Rasmus John Normand Frandsen

Rubini Maya Kannangara

Uffe Hasbro Mortensen

Majse Nafisi

Paiman Khorsand-Jamal

Kenneth Thermann Kongstad

Dan Stærk

Ulf Thrane

Kim Binderup

Mads Bennedsen

Björn Hamberger

Eirini Pateraki

Niels Bjerg Jensen

Morten Thrane Nielsen

Hilde Cornelijne Coumou

Morten Nørholm

Carl Jörg Bohlmann

Philipp Zerbe

A method for producing an octaketide derived aromatic compound of interest (e.g. carminic acid), wherein the method comprises (I): heterologous expression of a recombinantly introduced Type III polyketide synthase (PKS) gene encoding an octaketide synthase (OKS) to obtain non-reduced octaketide in vivo within the recombinant host cell and (II): converting in vivo the non-reduced octaketide of step (I) into a C-Caromatic compound of interest (e.g. carminic acid).

Use of octaketide synthases to produce kermesic acid and flavokermesic acid

A method for producing individual or libraries of tri- to pentadecaketide-derived aromatic compounds of interest by heterologous expression of polyketide synthase and aromatase/cyclase in a recombinant host cell.

Use of heterologous expressed polyketide synthase and small molecule foldases to make aromatic and cyclic compounds

The invention described materials and methods for producing oxidized 13R-MO, such as forskolin. In particular, the invention describes P450s involved in oxidation of 13R-MO including CYP76AH8, CYP76AH11, CYP76AH15, CYP76AH17, CYP71D381 and CYP76AH9. Host organisms expressing one or more of these P450s are useful in the production of oxidized 13R-MO.

Biosynthesis of forskolin and related compounds

Growing community of inventors

Berkeley, CA, United States of America

Johan Andersen-Ranberg