Maat Pharma

Institut National De La Recherche Agronomique

Institut National De La Sante Et De La Recherche Medicale

Assistance Publique-hopitaux De Paris

Université Paris—sud

Institut National De La Recherche Agronomique (inra)

Institut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement

Institut National De Recherche Pour L'agriculture

Université Pierre Et Marie Curie

Institut De Recherche Pour Le Developpement

Joël Dore

Carole Schwintner

Hervé Affagard

Catherine Juste

Fernanda Fonseca

Patricia Lepage

Jamila Faivre

Marion Darnaud

Emmanuelle Le Chatelier

Stanislav Ehrlich

Audrey Chapron

Nicolas Moniaux

Karine Clement

Olivier David

Hervé Blottiere

Sylvie Rabot

Christel Maillet

Jean-Daniel Zucker

A sampling method using a sampling device () comprising a container () comprising a body () consisting of a soft pouch, and a lid (), in which at least one among the container () and the lid () is provided with a discharging member () suitable for, in an open state, discharging at least one part of the gases contained in the internal space () of the body () of the container (), wherein the sampling method comprises the steps consisting in: —collecting a biological material in the body () of the container (), and assembling the lid () on the neck (IO) of the container (), —and placing an internal space () of the body () of the container () under anaerobic conditions by placing the discharging member () in the open state, by compressing the body () of the container () and by placing the discharging member () in a closed state.

Microorganism sampling method, microorganism sampling device and sampling kit comprising such a sampling device

The invention concerns a Reg3&#x3b1; polypeptide (also known as Hepatocarcinoma-Intestine-Pancreas/Pancreatitis Associated Protein (HIP/PAP)) for use in the protection of oxygen sensitive gram-positive bacteria, compositions comprising the polypeptide and their use. The inventors have shown that an increase in the concentration of the h Reg3&#x3b1; lectin into the gastrointestinal tract (GIT) lumen of hReg3&#x3b1;-transgenic mice induced significant changes in the composition of the gut microbiota, and dramatically improved host resistance to intestinal inflammation. hReg3&#x3b1; exerted a potent antioxidant activity on intestinal epithelial cells during colitis, and in particular the ROS scavenging activity, in particular, by promoting the survival of highly oxygen sensitive bacteria. Inventors also showed that h Reg3&#x3b1;-transgenic mice resist better to DSS-induced colitis after antibiotherapy. Thus, the invention concerns a Reg3&#x3b1; polypeptide for use in the protection of oxygen sensitive gram-positive bacteria, notably the Ruminococcaceae, such as, and/or the Lachnospiraceae, such as; pharmaceutical compositions comprising the polypeptide and their use; and the use of this polypeptide for promoting ex vivo growth of oxygen sensitive gram-positive bacteria. The Reg3&#x3b1; polypeptide may be used for preventing or treating microbiota-related disease and/or disorder, particularly selected from inflammatory bowel disease (IBD), colitis, gastrointestinal infections, irritable bowel syndrome and other gastrointestinal functional diseases, gastrointestinal tract cancer, metabolic syndrome and obesity, diabetes, liver diseases, allergic diseases, neurodegenerative diseases and psychological disorders.

Polypeptide for use in the protection of oxygen sensitive gram-positive bacteria

A sampling method using a sampling device () comprising a container () comprising a body () consisting of a soft pouch, and a lid (), in which at least one among the container () and the lid () is provided with a discharging member () suitable for, in an open state, discharging at least one part of the gases contained in the internal space () of the body () of the container (), wherein the sampling method comprises the steps consisting in: &#x2014;collecting a biological material in the body () of the container (), and assembling the lid () on the neck () of the container (), &#x2014;and placing an internal space () of the body () of the container () under anaerobic conditions by placing the discharging member () in the open state, by compressing the body () of the container () and by placing the discharging member () in a closed state.

The present invention relates to a method for preparing a fecal 5 microbiota sample of a donor subject. Said method includes the following steps: a) collecting at least one fecal microbiota sample from the donor subject; b) within a period of less than 5 minutes after collecting the sample, placing said sample obtained in step a) in an oxygen-tight collection device; c) mixing the sample obtained in step b) with at least one aqueous saline 10 solution containing at least one cryoprotectant and/or a filling agent; d) optionally, filtering the mixture obtained in step c), in particular by means of a filter comprising pores having a diameter of less than or equal to 0.7 mm, preferably less than or equal to 0.5 mm; and e) storing the mixture obtained in step c) or d) by freezing said mixture at a temperature 15 of between &#x2212;15&#xb0; C. and &#x2212;100&#xb0; C. Steps b) to e) are carried out in anaerobiosis.

Method of preparing a faecal microbiota sample

Method for lyophilisation of a simple of fecal microbiota. The present invention relates to a method for lyophilisation of a sample of fecal microbiota from a donor subject, comprising the following steps: A) mixing of a sample of fecal microbiota from a donor subject with a diluent selected from polyols, disaccharides to pentasaccharides, maltodextrins and mixtures thereof, and B) freezing the mixture obtained in A) at a temperature of less than &#x2212;50&#xb0; C., preferably of between &#x2212;70&#xb0; C. and &#x2212;100&#xb0; C., followed by the lyophilisation thereof.

Method of lyophilization of a sample of faecal microbiota

Growing community of inventors

Vitry-sur-Seine, France

Joël Dore