Abberior Instruments Gmbh

Joern Heine

Matthias Reuss

Lars Kastrup

Benjamin Harke

Gerald Donnert

Haugen Mittelstaedt

A fluorescence microscope () includes a sample illumination beam path including a source () for illumination light, a first wave front modulator () for providing the focused illumination light () with a central intensity minimum, a beam splitter () and a second adjustable wave front modulator () arranged in a pupil plane () of an objective (). A first detection beam path section including the second wave front modulator () and a telescope () and ending at the beam splitter () coincides with the sample illumination beam path. A separate second detection beam path section includes a detector () for luminescence light from a sample. The telescope () images a first pupil () formed in the pupil plane () in a smaller second pupil (), and transfers a beam of the illumination light () collimated in the second pupil () into an expanded beam collimated in the first pupil ().

Fluorescence microscope with stabilized adjustment and group of components and module for upgrading a fluorescence microscope

For setting a laser-scanning fluorescence microscope to a correct alignment in which an intensity maximum of excitation light and an intensity minimum of fluorescence inhibition light coincide in a focal area of an objective lens, a structure in a sample marked with a fluorescent dye is scanned with the intensity maximum of the excitation light to generate first and second pictures of the sample, the first picture corresponding to a higher and the second picture corresponding to a lower intensity of the fluorescence inhibition light. A spatial offset of a first image of the structure in the first picture with regard to a second image of the structure in the second picture is calculated; and the intensity maximum of the excitation light is shifted with regard to the intensity minimum of the fluorescence inhibition light in the direction of the offset calculated to set the microscope to the correct alignment.

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Goettingen, Germany

Joern Heine