Hoffmann-La Roche Inc.

Joerg-Thomas Regula

Christian Klein

Wolfgang Schaefer

Birgit Bossenmaier

Hubert Kettenberger

Klaus-Peter Kuenkele

Claudio Sustmann

Manfred Schwaiger

Michael Molhoj

Joerg Moelleken

Christian Gassner

Manuel Endesfelder

Herein is reported a method for determining the binding interaction with a multimeric antigen of an antibody of the human IgG1 subclass that has at least two binding sites specifically binding to the antigen comprising the steps of 1) determining the binding affinity of the antibody for the multimeric antigen, and 2) incubating a mixture comprising the antibody and a polypeptide that is derived from lysine-gingipain ofunder conditions and for a time sufficient to cleave the antibody into Fabs and Fc-region, and determining the binding affinity of the Fabs of the antibody for the multimeric, whereby the binding affinity of the antibody to the multimeric antigen to be affinity-driven if the binding affinity determined in both steps are comparable and to be avidity-driven if the binding affinity determined in both steps are different.

Method for determining the in vivo interaction mode

The present invention relates to antigen binding proteins comprising two Fc parts, methods for their production, pharmaceutical compositions containing said antigen binding proteins, and uses thereof.

Nucleic acid encoding dual Fc antigen binding proteins

Dual FC antigen binding proteins

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Munich, Germany

Joerg-Thomas Regula