Miltenyi Biotec B.v. Co., Kg

Joerg Mittelstaet

Stefan Miltenyi

Andrew Kaiser

Volker Huppert

Thomas Schaser

Rupert Handgretinger

Nicole Cordes

Bettina Kotter

Bianca Heemskerk

Christian Seitz

Peter Lang

Patrick Schlegel

Brian Webster

The present invention provides a composition comprising i) a pseudotyped retroviral vector particle or virus-like particle thereof comprising a) one envelope protein with antigen-binding activity, wherein said envelope protein is a recombinant protein that does not interact with at least one of its native receptor(s) and is fused at its ectodomain to a polypeptide comprising an antigen binding domain specific for a tag of a tagged polypeptide, and wherein said envelope protein is protein G, HN or H derived from the Paramyxoviridae family, and b) one envelope protein with fusion activity derived from the Paramyxoviridae family, and ii) said tagged polypeptide, wherein said tagged polypeptide binds specifically to an antigen expressed on the surface of a target cell, thereby transducing the target cell with said retroviral vector particle or thereby inducing uptake of the virus-like particle into the target cell. A pharmaceutical composition thereof and an in vitro method for transduction of targets cells with said vector particle are also disclosed.

Adapter-based retroviral vector system for the selective transduction of target cells

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Cologne, Germany

Joerg Mittelstaet