Carl Zeiss Microscopy Gmbh

Carl Zeiss Microimaging Gmbh

Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.V.

Carl Zeiss Jena Gmbh

Joerg Engel

Joerg Steinert

Stefan Wilhelm

Ralf Engelmann

Ulrich Meisel

Bernhard Zimmermann

Joerg-Michael Funk

Thomas Egloff

Nico Presser

Frank Klemm

Volodymyr Kudryavtsev

Andreas Kuehm

Christopher Power

Frank Hecht

Stanislav Kalinin

Carsten Wehe

Stefan Brandys

Steffen Suepple

Hardy Thomas Koebe

Markus Turber

Daniel Mauch

Alexander Kolarow

Bernhard Goetze

Fabian Hermann

A method for high-resolution scanning microscopy of a sample, wherein the sample is illuminated with illumination light such that the illumination light is focused at a point in or on the sample into an illumination spot. The point is imaged into a diffraction image onto an area detector having detector elements. The area detector has a spatial resolution that resolves a diffraction structure of the diffraction image. The sample is here scanned line-wise in a grid made of rows and columns by displacing the point relative to the sample into different scanning positions with an increment width that is smaller than the diameter of the illumination spot. The area detector is read, and an image of the sample is generated from the data of the area detector and from the scanning positions assigned to said data, said image having a resolution that is increased beyond a resolution limit for imaging.

Method for high-resolution scanning microscopy

The invention is based on the object of providing a particularly reliable closed-loop control for a scanner. According to various examples, this object is achieved by an analysis of a system deviation in the frequency space. By way of example, an input signal, which is indicative of a time dependence of the system deviation between an ACTUAL pose and a TARGET pose of a deflection unit of the scanner, can be expanded into a multiplicity of error components and a plurality of frequencies. Then, a corresponding correction signal component can be determined for each of the multiplicity of error components. By way of example, such techniques can be used in a laser scanning microscope.

Closed-loop control of a scanner with frequency-space analysis of a system deviation

Forwarding devices and corresponding methods are provided in which a plurality of input data streams are distributed among a plurality of output data streams on the basis of synchronization marking. One example method of forwarding data includes receiving a plurality of input data streams, where at least some of the input data streams include synchronization markers indicating which data of the input data streams are to be output synchronously, in one or more common time segments. Further included in the method is distributing the data to be output synchronously among a plurality of output data streams on the basis of the synchronization markers. The distributing is carried out in such a way that data which, according to the synchronization markers, are to be transmitted in a common time segment are provided in the same time segment in all the output data streams to which the data are to be assigned.

Method and apparatus for forwarding data

A method for generating a control signal is provided. The method includes the steps of decomposing a desired movement into two partial movements which are separately equalized, and the desired control signal is obtained by summing up the corrected components. The first movement is a slowly (mostly linear) changing long-period (period T) movement, and the second movement is a short-period (period T) movement, wherein the period Tis substantially longer than the period T. The movements have to a large extent opposing temporal derivations which are nevertheless equal in magnitude so that their sum has a time derivative that is zero. In addition, a method is provided for operating a scanning unit periodically displaceable in an infeed direction by an infeed distance.

Method for generating a control function and method for operating a scanning unit

A peripheral interface for use with a control computer and a peripheral device. The peripheral interface has a controller receiving an input data stream from the control computer and delivering an output data stream to the peripheral device, the controller obtaining an instruction from the input data stream for a modification of the output data stream. Prior art devices transfer data streams for peripheral devices blockwise by means of DMA using peripheral interfaces. In conventional peripheral interfaces, a burdensome real-time operating system must be used on the control computer in order have a sufficiently short reaction time to bring about a continuous, uninterrupted data stream. The invention achieves the object using a non-real-time operating system. A data stream is generated in the control computer, comprising control data (B) for the peripheral device and a segment (SOS) having spare control data to be output in case of a break in the data stream, and a leading spare data marking, particularly a jump command (JMP) past the spare control data. The data stream is received in the peripheral interface, and output to the peripheral device, wherein an instruction for a modification of the output of the data stream is identified in the received data stream, and the data stream is modified for output according to the identified instruction.

Peripheral interface, data stream, and data transfer method

In a method for correcting a control of an optical scanner () which has a beam deflecting element () for deflecting a beam of optical radiation and a drive unit () for moving the beam deflecting element (), said drive unit deflecting a beam of optical radiation directed at the beam deflecting element () according to a predetermined target movement using at least one parameter and/or a transfer function, preferably optical, said parameter or transfer function being used to control or regulate the system. In a determination step at least one current value of a drive unit transfer function that reproduces the response of the drive unit () to a predetermined target movement or a change in a target movement is ascertained for at least one frequency, and in a correction step at least one parameter and/or the transfer function is corrected as a function of the current value of the drive unit transfer function.

Method for correcting a control of an optical scanner and the optical scanner

User interface for computer screen

Process for observing at least one sample region with a light raster microscope by a relative movement between the illumination light and sample via a first scanner along at least one scanning axis essentially perpendicular to the illumination axis wherein several illuminated sample points lie on a line and are detected simultaneously with a spatially resolving detector. At an angle to the plane of the relative movement, a second scanner is moved and an image acquisition takes place by coupling the movement of the first and second scanners and a three-dimensional sampling movement being done by the illumination of the sample. The second scanner is coupled to the movement of the first scanner such that straight and/or curved lines and/or plane and/or curved surfaces are scanned which are extended along at least one scanning direction of the first scanner as well as along the scanning direction of the second scanner.

Process for the observation of at least one sample region with a light raster microscope with linear sampling

Process for the observation of at least one sample region with a light raster microscope by a relative movement between the illumination light and sample via a first scanner along at least one scanning axis essentially perpendicular to the illumination axis wherein at an angle to the plane of the relative movement, preferably perpendicular thereto a second scanner is moved and an image acquisition takes place by the movement of the first and second scanners being coupled and a three-dimensional sampling movement being done by the illumination of the sample wherein the second scanner is coupled to the movement of the first scanner in such a manner that straight and/or curved lines and/or plane and/or curved surfaces are scanned which are extended along at least one scanning direction of the first scanner as well as along the scanning direction of the second scanner.

Process for the observation of at least one sample region with a light raster microscope

Process for the observation of at least one sample region with a light raster microscope by a relative movement between the illumination light and sample via first scanning means along at least one scanning axis essentially perpendicular to the illumination axis wherein the illumination light illuminates the sample in parallel at several points or regions and several points or regions are detected simultaneously wherein at an angle to the plane of the relative movement, preferably perpendicular thereto, second scanning means are moved and an image acquisition takes place by the movement of the first and second scanning means being coupled and a three-dimensional sampling movement being done by the illumination of the sample wherein the second scanning means are coupled to the movement of the first scanning means in such a manner that straight and/or curved lines and/or plane and/or curved surfaces are scanned which are extended along at least one scanning direction of the first scanning means as well as along the scanning direction of the second scanning means.

Process for the observation of at least one sample region with a light raster microscope with light distribution in the form of a point

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Weida, Germany

Joerg Engel