Promega Corporation

Joel R Walker

Mary P Hall

Thomas A Kirkland

Thomas Machleidt

Anton Shakhmin

Wenhui Zhou

Keith V Wood

Poncho Meisenheimer

Matthew B Robers

Rachel Friedman Ohana

Harry Tetsuo Uyeda

Christopher Todd Eggers

Horacio Lazaro

Hui Wang

Brock Binkowski

Chad Zimprich

Nidhi Nath

Ce Shi

Sarah Duellman

Sergei Saveliev

Sergiy Levin

Min Zhou

Roderick G Flemming

Zhiyang Zeng

Robert Kargbo

Valerie Ressler

Jun Zhang

Jean Osterman

Prabin Rai

David M Jenkins

Yusuke Nakamura

Thomas Kirland

Feryan Ahmed

Raymond Huntley

Robert B Kargbo

Polivina Jolicia F Gauuan

Wenge Cui

Described are substituted imidazo[1,2-a]pyrazine compounds, which are coelenterazine analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays. Also described are methods from making the compounds, such as a method using aminopyrazine acetophosphonates as synthesis intermediates.

Substituted imidazo[1,2-α]pyrazines as luciferase substrates

Compounds that may selectively inhibitluciferase-derived bioluminescent complexes, e.g., NanoBiT® bioluminescent complex, are disclosed as well as compositions and kits comprising the compounds, and methods of using the compounds.

Inhibitors of oplophorus luciferase-derived bioluminescent complexes

Provided herein are surfactant compounds that can be used to aid in preparation of protein samples for analysis, for example by mass spectrometry.

Acid-labile surfactants

Described are processes for preparing substituted imidazo[1,2-a]pyrazine compounds of formula (I): by reacting a compound of formula (xi): with carbonyldiimidazole to form the compound of formula (I).

Process for preparing substituted imidazo[1,2-&#x3b1;]pyrazines

Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.

Coelenterazine analogues

Compounds that may inhibit-derived luciferases are disclosed as well as compositions and kits comprising the compounds and methods of using the compounds.

Nanoluc suicide substrates

Compounds that may selectively inhibitluciferase-derived bioluminescent complexes, e.g., NanoBiT&#xae; bioluminescent complex, are disclosed as well as compositions and kits comprising the compounds, and methods of using the compounds.

Cell impermeable coelenterazine analogues

Described are substituted imidazo[1,2-a]pyrazine compounds, which are coelenterazine analogues, kits comprising the compounds, and methods of using the compounds for the detection of luminescence in luciferase-based assays. Also described are methods of making the compounds, such as a method using aminopyrazine acetophosphonates as synthesis intermediates. The substituted imidazo[1,2-a]pyrazine compounds include compounds of formula (I): 

Substituted imidazo[1,2-a]pyrazines as luciferase substrates

Compounds that may inhibit-derived luciferases are disclosed as well as compositions and kits comprising the compounds, and methods of using the compounds.

Functionalized NANOLUC inhibitors

Described are substituted imidazo[1,2-a]pyrazine compounds, which are coelenterazine analogues, kits comprising the compounds, and methods of using the compounds for the detection of luminescence in luciferase-based assays. Also described are methods from making the compounds, such as a method using aminopyrazine acetophosphonates as synthesis intermediates.

Thienopyrrole compounds that may inhibit-derived luciferases are disclosed, as well as compositions and kits comprising the thienopyrrole compounds, and methods of using the thienopyrrole compounds.

Thienopyrrole compounds and uses thereof

Growing community of inventors

San Luis Obispo, CA, United States of America

Joel R Walker