National Institutes of Health, a Component of the US Dept. of Health & Human Services

University of Delaware

The Johns Hopkins University

The Penn State Research Foundation

National Institutes of Health

Kennedy Krieger Institute, Inc.

Joel Patrick Schneider

Darrin J Pochan

Michael T McMahon

Xiaolei Song

Gerald Brandacher

Scott Durum

Scott Walsh

Scott Hammond Medina

Julie Hixon

Nikita Oskolkov

Bulent Ozbas

Daphne A Salick

Juliana Kretsinger

Lisa Ann Butterick

Karthikan Rajagopal

Wenqing Li

Steven Tau

Daniel Smith

Kannie Wai-Yan Chan

Yuji Yamada

Gabriel Brat

Stephen Miller

Caroline Andrews

Johanna Grahammer

Giorgio Raimondi

Poulami Majumder

Chuong Hoang

Michael Christopher Giano

Anand Singh

The present invention is directed to peptisomes, including nanopeptisomes, which have a perfluorocarbon liquid core containing a perfluorocarbon liquid and a cargo, such as a therapeutically active agent, dispersed in the perfluorocarbon. liquid, and a plurality of amphiphilic peptide molecules surrounding the perfluorocarbon liquid core, wherein the amphiphilic peptide is represented by Formula (I) HB-CL-HP wherein HB is a fluorinated hydrophobic block, such as a fluorinated hydrophobic amino acid sequence, CL is a cross-linking motif, and HP is a hydrophilic amino acid sequence. The present invention is also directed to methods of use of the amphiphilic peptides and peptisomes, such as nanopeptisomes, to deliver a cargo, such as a therapeutically active agent, to a cell, Q wherein the cell may be in vitro, ex vivo, or in vivo.

Ultrasound-sensitive peptide particles for spatially resolved molecule delivery

This disclosure provides novel peptide hydrogels containing encapsulated nanoparticles comprising nucleic acid molecules (such as miRNA) that can undergo multiple gel-to-solution (gel-sol) and solution-to-gel (sol-gel) phase transitions, and their use, such as for controlled delivery of nucleic acid molecules to a subject.

Nanoparticle-hydrogel composite for nucleic acid molecule delivery

This disclosure provides novel anionic amphiphilic &#x3b2;-hairpin peptides that self-assemble under appropriate conditions to form a reversible gel-sol hydrogel that can be used, for example, to readily deliver protein therapeutics and cells by injection to a target location in a subject.

Peptide hydrogels and use thereof

The present invention provides novel hydrogels through peptides, which are designed to self-assemble and produce magnetic resonance (MR) contrast through chemical exchange saturation transfer (CEST). The location and integrity of these gels could consequently be tracked using MR imaging. The self-assembly of the peptides into hydrogels can be brought about by a change in pH, ionic strength, temperature, and concentration of ions.

Peptide hydrogels generating chemical exchange saturation transfer MRI contrast and uses thereof

This disclosure provides novel hydrogels that can undergo multiple gel-sol transitions and methods of making and using such hydrogels, particularly in anastomosis procedures. The peptide hydrogels comprising a fibrillar network of peptides that are in an amphiphilic &#x3b2;-hairpin conformation. The peptides comprise photo-caged glutamate residues with a neutral photocage that can be photolytically selectively uncaged to disrupt the fibrillar network and trigger an irreversible gel-sol phase transition of the hydrogel. Isolated peptides for making the disclosed hydrogels are provided, as are methods of using the peptide hydrogels in anastomosis procedures.

Hydrogels and use thereof in anastomosis procedures

A peptide comprising the sequence VKVKVRVKVPPTKVKVRVKV-NHforms a hydrogel which has the ability to shear-thin and recover. The hydrogel, both before and after shear-thinning, is capable of killing bacteria, including methicillin-resistant(MRSA).

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The present invention provides novel hydrogels and methods of making and using such hydrogels. The present invention provides hydrogels that may be formed by the self-assembly of peptides in solution. Such self-assembly may be brought about by a change in one or more characteristics of the solution. Characteristics of the solution that may be changed include pH, ionic strength, temperature, and concentration of one or more specific ions. In addition, hydrogels of the invention may be disassembled by changing one or more characteristic of the hydrogel such as pH, ionic strength, temperature, and concentration of one or more specific ions.

Hydrogels and uses thereof

Growing community of inventors

Frederick, MD, United States of America

Joel Patrick Schneider