Thermo Fisher Scientific Gmbh

Pierce Biotechnology, Inc.

Joel Louette

John Charles Rogers

Scott M Peterman

Bruno Domon

Elodie Duriez

Greg Hermanson

Ryan Daniel Bomgarden

Bhavinkumar Patel

Quantitation of analytes, including but not limited to peptides, polypeptides, and proteins, in mass spectrometry using a labeled peptide coupled to a reporter, and a universal reporter.

Analyte mass spectrometry quantitation using a universal reporter

A method for absolute protein or peptide quantitation by mass spectroscopy. A sample containing a protein or peptide of interest is prepared for mass spectroscopy analysis. The sample is subjected to mass spectroscopy analysis at low resolution whereby a single additive mass spectroscopy peak is obtained, then is subjected to high resolution mass spectroscopy analysis whereby a plurality of mass spectroscopy peaks are obtained. The intensity of each of the plurality of mass spectroscopy peaks is quantitated either by comparison to an internal standard set, or by using a standard curve generated for each isotopologue set. Quantitation using a standard curve enhances quantitation across a dynamic range of analyte.

Absolute quantitation of proteins and protein modifications by mass spectrometry with multiplexed internal standards

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Ulm, Germany

Joel Louette