Streck, Inc.

Creighton University

Joel Lechner

Matthew R Kreifels

Nancy D Hanson

Stacey Morrow

Stephanie Cossette

Maria Torres-Gonzalez

Randy Fowler

Laura R Porter

Cathy Scheer

Christopher Connelly

Kausik Das

Gary D Krzyzanowski

Stephanie Wigginton

Chris Connelly

Maria Torres

Assays and methods for detecting resistance to beta-lactam antibiotics including detection of multiple β-lactamase family specific gene targets by polymerase chain reaction or microarray. One or more kits including primers and/or probes for identification of β-lactamase genes selected from the group consisting of one or more of the following: MOX-like, FOX-like, ACC-like, ACT/MIR-like, CMY-2-like, DHA-like, CTX-M-14-like, CTX-M-15-like, VIM-like, NDM-like, IMP-like, KPC-like, and OXA-48-like, OXA-51-like, OXA-143-like, OXA-58-like, OXA-23-like, OXA-24/40-like, TEM-like, and SHV-like. A kit may also include one or more primers and/or probes for the identification a non-beta lactamase gene family which confers antibiotic resistance, such as the MCR-1 gene.

Assays and methods for determining microbial resistance

Assays and methods for detecting resistance to beta-lactam antibiotics including detection of multiple &#x3b2;-lactamase family specific gene targets by polymerase chain reaction or microarray. One or more kits including primers and/or probes for identification of &#x3b2;-lactamase genes selected from the group consisting of one or more of the following: MOX-like, FOX-like, ACC-like, ACT/MIR-like, CMY-2-like, DHA-like, CTX-M-14-like, CTX-M-15-like, VIM-like, NDM-like, IMP-like, KPC-like, and OXA-48-like, OXA-51-like, OXA-143-like, OXA-58-like, OXA-23-like, OXA-24/40-like, TEM-like, and SHV-like. A kit may also include one or more primers and/or probes for the identification a non-beta lactamase gene family which confers antibiotic resistance, such as the MCR-1 gene.

By way of summary, the present invention meets some or all of the above needs by providing in a first aspect a method comprising contacting red blood cells in a suspension medium containing mannose under conditions such that the concentration of S-Alc glycated hemoglobin is increased to greater than about 6 percent by weight of the hemoglobin in the red blood cells. Preferably the suspension medium is an aqueous suspension medium. In preferred embodiments, the method is performed at about ambient temperature, 18&#xb0; C. to about 23&#xb0; C.

Method of preparing controls for glycated hemoglobin S-A1c derived from healthy blood cells

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Omaha, NE, United States of America

Joel Lechner