Dow Agrosciences LLC

Agrigenetics, Inc.

Corteva Agriscience LLC

Joel Jay Sheets

Thomas Meade

Kenneth Edwin Narva

Aaron Todd Woosley

Stephanie L Burton

Sek Yee Tan

Nicholas P Storer

Timothy Denver Hey

Todd P Glancy

Sarah E Worden

Diaa Alabed

Karen C McLaughlin

Justin Michael Lira

Sean Michael Russell

Krishna M Madduri

Gaofeng Lin

Jon Christopher Mitchell

Patricia C Apel-Birkhold

Janna Mai Armstrong

Jianquan Li

Audrey Jane Etter

Kristin J Fencil

Ze-Sheng Li

Robin Leola Thompson

Carla Nanette Yerkes

Yuanming Zhu

David Anthony Demeter

Donald J Merlo

Gerald Bryan Watson

Jim Xinpei Huang

Zoltan Laszlo Benko

Lowell D Markley

James Michael Gifford

James Edwin Dripps

Laura Lee Karr

Peter Biagio Anzeveno

Paul Allen Neese

Jack Geno Samaritoni

Leonard P Dintenfass

Premchand Gandra

James Todd Pechacek

Qihua C Cai

W Randal Erickson

Ted Letherer

Haley Mowery

Gerrit Deboer

Susan Zabik

Christian Thomas Schobert

Haley Jo Moon

Carl Vincent DeAmicis

Ignacio Mario Larrinua

Scott Bevan

Steven Lee Evans

Meghan Frey

James Edward King

Ryan M Lee

James M Hasler

Tristan E Coram

Weiting W Ni

Carl DeAMICIS

Meghan L F Frey

Carrie Lynn Rau Schmidt

Carrie Schmidt

James E King

Stephanie Burton

Karen C Mclaughlin

Steven L Evans

Stephenie L Burton

Leonard Paul Dintenfass

Gerrit Jan Deboer

Susan Erhardt Zabik

Lowell Dean Markley

Yuamning Zhu

The subject invention relates in part to the surprising discovery that CryDa is active against corn earworm (CEW),(Boddie). Methods for using CryDa in transgenic plants to prevent serious crop damage is described. Leaf and silk bioassays using transgenic maize expressing full length, core toxin region or chimeric CryDa demonstrated good insect protection against CEW larvae damage.

Cry1D for controlling corn earworm

The subject invention relates in part to the surprising discovery that Cry1Da is active against corn earworm (CEW),(Boddie). Methods for using Cry1Da in transgenic plants to prevent serious crop damage is described. Leaf and silk bioassays using transgenic maize expressing full length, core toxin region or chimeric Cry1Da demonstrated good insect protection against CEW larvae damage.

DIG-657 vegetative insecticidal toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed. The invention includes DIG-657 variants, fragments and analogs.

Vegetative insecticidal proteins useful for control of insect pests

The subject invention concernsmodified Cry1Ca insecticidal toxins and the polynucleotide sequences which encode these toxins. Uses in transgenic plants are described as are methods for protecting crops from insect pest damage.

Modified Cry1Ca toxins useful for control of insect pests

The subject invention includes methods and plants for controlling European corn borer, said plants comprising a Cry1Ab insecticidal protein and a DIG-3 insecticidal protein to delay or prevent development of resistance by the insect.

Use of DIG3 insecticidal crystal protein in combination with cry1Ab for management of resistance in european cornborer

CRY1D for controlling corn earworm

The subject invention includes methods and plants for controlling fall armyworm lepidopteran insects, said plants comprising a Cry1Da insecticidal protein and a Cry1Ca insecticidal protein, and various combinations of other proteins comprising this pair of proteins, to delay or prevent development of resistance by the insects.

Use of Cry1Da in combination with Cry1Ca for management of resistant insects

The subject invention includes methods and plants for controlling European corn borer and/or fall armyworm insects, said plants comprising a Cry1Ab insecticidal protein and a Cry1Be insecticidal protein, and various combinations of other proteins comprising this pair of proteins, to delay or prevent development of resistance by the insects.

Use of Cry1Ab in combination with Cry1Be for management of resistant insects

The subject invention includes methods and plants for controlling lepidopteran insects. The plants comprise a Cry1Fa insecticidal protein and a Cry1Ca insecticidal protein, in combination, to delay or prevent development of resistance by the insect(s).

Combined use of CRY1Ca and CRY1Fa proteins for insect resistance management

The subject invention relates in part to stacking Cry1Be toxins along with Cry1Fa toxins to prevent insects from developing resistance towards either toxin by itself. As discussed in more detail herein, the subject pair of proteins is a particularly advantageous combination, as no other pair of proteins is known to provide high levels of control and non-cross-resistant activity against both(FAW) and(ECB) insects. This dual, non-cross-resistant activity is also advantageous because it can reduce the number of proteins/genes needed to target these insects with multiple, non-cross-resistant proteins. This can reduce or eliminate the need for refuge acreage. Accordingly, the subject invention also relates generally to using four genes to provide three proteins for non-cross-resistant control of a first insect, and three proteins for non-cross-resistant control of a second insect. In preferred embodiments, the targeted insects are FAW and ECB.

Insect resistance management with combinations of Cry1Be and Cry1F proteins

The subject invention includes methods and plants for controlling fall army worm insects, said plants comprising a Cry1Da insecticidal protein and a Cry1Be insecticidal protein, and various combinations of other proteins comprising this pair of proteins, to delay or prevent development of resistance by the insects.

Use of Cry1Da in combination with Cry1Be for management of resistant insects

The invention is drawn to a method for purifying membrane-bound proteins expressed in recombinant insect cells using N-laurosarcosine. The invention is particularly suited for expressing cadherin-type receptors cloned from, European Corn Borer, and expressed in Sf9 insect cells. The method is optionally adapted for use with 6-his tag proteins.

Methods for purifying insect membrane-bound receptor proteins from recombinant production hosts

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Zionsville, IN, United States of America

Joel Jay Sheets