Aviva Biosciences Corporation

Joe Ouyang

Jing Cheng

Xiaobo Wang

Lei Wu

Junquan Xu

George T Walker

Mingxian Huang

Guoliang Tao

Douglas T Yamanishi

Jia Xu

Paul G Hujsak

Ping Lin

Sara F Snyder

Charina Schmitigal

Elizabeth Kwok

The present invention recognizes that diagnosis and prognosis of many conditions can depend on the enrichment of rare cells, especially tumor cells, from a complex fluid sample such as a blood sample. In particular, the present invention is directed to methods and compositions for detecting a non-hematopoietic cell, e.g., a non-hematopoietic tumor cell, in a blood sample via, inter alia, removing red blood cells (RBCs) from a blood sample using a non-centrifugation procedure, removing white blood cells (WBCs) from said blood sample to enrich a non-hematopoietic cell, if any, from said blood sample; and assessing the presence, absence and/or amount of said enriched non-hematopoietic cell.

Methods and compositions for detecting non-hematopoietic cells from a blood sample

The present invention includes methods of enriching rare cells, such as cancer cells, from biological samples, such as blood samples. The methods include performing at least one debulking step on a blood sample and selectively removing at least one type undesirable component from the blood sample to obtain a blood sample that is enriched in a rare cell of interest. In some embodiments magnetic beads coupled to specific binding members are used to selectively removed components.

Methods and compositions for separating rare cells from fluid samples

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San Diego, CA, United States of America

Joe Ouyang