Albert-ludwigs-universitaet Freiburg

Albert-ludwigs-universitat Freiburg

Biocopy Gmbh

Jochen Hoffmann

Guenter Roth

Roland Zengerle

Felix Von Stetten

Günter Roth

The invention relates to a method for performing a biochemical or chemical reaction for an isolated, spatially separated amplification of sample fragments during a simultaneous immobilization and spatial arrangement of the sample fragments and reaction products, the amplification products, on one or more suitable solid phases for subsequent derivatizations.

Method for the spatial arrangement of sample fragments for amplification and immobilization for further derivatizations

The invention relates to a method for detecting at least one target molecule and/or product molecule, wherein reaction components are provided in defined regions of a base surface and/or covering device. At least two defined regions with different reaction components are provided, and an amplification reaction is carried out. The invention also relates to a device for carrying out said method.

Multiplexed digital PCR

A method of producing a replicate or derivative of an array of molecules, the array having a spatial arrangement of separate samples of molecules, includes creating, for each sample, at least one spatially limited effective area which is separate from the effective areas of the other samples, a surface, provided with a binding adapter or binding properties, of a carrier bordering on the effective areas. The molecules are amplified by means of amplifying agents in the effective areas for creating replicates or derivatives of the samples. The replicates or derivatives of the samples are bound to the carrier by means of the binding adapter or the binding properties, so that a spatial arrangement of the replicates or derivatives of the samples on the carrier corresponds to the spatial arrangement of the samples in the array. The carrier having the copies of the samples is removed from the array.

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Freiburg, Germany

Jochen Hoffmann