Rwth Aachen

Adolf Kühner Ag

Rheinisch-westfälisch Technische Hochschule (rwth) Aachen

Rheinisch-westfalische Technische Hochschule Aachen

M2p-labs Gmbh

Rwth Aachen University

Jochen Büchs

Wilfried Mokwa

Frank Kensy

Mathias Schumacher

Doris Klee

Burkhard Corves

Uwe Schnakenberg

Uwe Conrath

Barbara Dittrich

Mirko Hofmann

Andreas Buchenauer

Oliver Paquet-Durand

Bernd Hitzmann

Georg Wandrey

Tobias Ladner

Markus Samorski

Matthias Funke

Ingo Klammer

Wolf Klöckner

Markus Jeude

Britta Schillheim

Carsten MÜLLER

A method for measuring process parameters in liquid cultures in a plurality of microreactors of at least one microtiter plate includes continuously agitating the liquid cultures using an orbital agitator at least until the reaction is completed in all the microreactors. In order to allow process parameters also of such substances which themselves do not have any fluorescence activity to be measured with relatively low complexity and within a short time, 2D fluorescence spectra are recorded in a plurality of in particular different liquid cultures in the microreactors of agitated microplates. A device for carrying out the method is also disclosed.

Method and device for measuring process parameters in liquid cultures

An orbital shaker with a balancing device that automatically and passively compensates, without supply of external energy, forces and moments which are caused by imbalance and arise during operation, in particular as a result of a possible variable loading (mass, geometry of the containers, properties of the contents) and variable operating parameters (speed of rotation and shaking radius). The balancing device can be designed such that only the unbalance forces (static balancing) and/or the unbalance moments (dynamic balancing) are compensated.

Orbital shaker with balancing device

The invention relates to a method and a device for the recording of process parameters of reaction fluids in several microreactors which are continuously agitated, at least until the termination of the reaction in all the microreactors. The process parameters in the microreactor are recorded during the reaction by means of at least one sensor optical system. According to the invention, the reliability of the method may be increased, whereby, during the recording of the value of a process parameter, for example, on recording an instantaneous value of the auto-fluorescence of the reaction fluids, the sensor optical system is held stationary. The relative movement of the agitated microreactor and each sensor optical system thus produced is not problematical when the electromagnetic radiation from each sensor optical system is introduced exclusively into one of the microreactors concerned during the recording of the process parameter in said microreactor and the radiation emitted from the reaction fluid is only incident on the sensor of the corresponding sensor optical system.

Method and device for recording process paramaters of reaction fluids in several agitated microreactors

The invention relates to a microtiter plate and use thereof for conducting fermentation under fed-batch conditions. In order to produce a microtiter plate which permits screening under fed-batch conditions, the invention proposes that the cavities () of the microtiter plate according to the invention be filled with a culturing fluid and nutrient solution and be designed in such a way that each of the cavities () of the microtiter plate which is filled with nutrient solution is connected by a channel () to at least one other further cavity () of the microtiter plate which is filled with a culturing fluid. A diffusion barrier () arranged in the material permeable channel () controls the kinetics of the material transfer of nutrients from the cavity containing the nutrient solution to the cavities containing the culturing fluid.

Microtiter plate and use thereof

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Aachen, Germany

Jochen Büchs