Cornell Research Foundation Inc.

Federacion Nacional De Cafeteros De Colombia

Boyce Thompson Institute for Plant Research

Jocelyn Kenneth Rose

Julia Vrebalov

Ricardo Acuna Zornosa

Tal Isaacson-Lustig

Ricardo Acuña

Disclosed is an isolated nucleic acid molecule comprising a nucleotide sequence that is at least 90% identical to a nucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO 4, wherein the protein has an aspartic acid (Asp) at amino acid position 106 Transgenic plants comp&#x3c0;sing the nucleic acid have reduced or delayed softening and detenoration of fruit compared to wild-type plants Methods of genetic screening for plants having reduced or delayed softening and detenoration of fruit, isolated oligonucleotides, and a method of imparting to a plant the dfd trait are also disclosed.

Delayed fruit deterioration allele in plants and methods of detection

The present invention relates to an isolated &#x3b2;-mannanase protein having an amino acid sequence which is 90% similar to the amino acid sequence of SEQ ID NO:1, as well as isolated polynucleotides encoding the &#x3b2;-mannanase protein, and isolated expression systems and host cells containing the polynucleotides. The present invention also relates to a method of recombinantly producing &#x3b2;-mannanase protein. Also disclosed is a method of degrading mannans and polysaccharides in plant material, which involves providing plant material and contacting the plant material with the &#x3b2;-mannanase protein of the present invention under conditions effective to degrade mannans and polysaccharides in the plant material.

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The present invention relates to an isolated &#x3b2;-mannanase protein having an amino acid sequence which is 90% similar to the amino acid sequence of SEQ ID NO: 1, as well as isolated polynucleotides encoding the &#x3b2;-mannanase protein, and isolated expression systems and host cells containing the polynucleotides. The present invention also relates to a method of recombinantly producing &#x3b2;-mannanase protein. Also disclosed is a method of degrading mannans and polysaccharides in plant material, which involves providing plant material and contacting the plant material with the &#x3b2;-mannanase protein of the present invention under conditions effective to degrade mannans and polysaccharides in the plant material.

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Ithaca, NY, United States of America

Jocelyn Kenneth Rose