University of California

Lawrence Livermore National Security, LLC

Onyx Pharmaceuticals, Inc.

Joanna Albala

Stephen M Lane

Dennis L Matthews

Matthew A Coleman

Robin Clark

Deanna Lynn Thompson

Ken Franke

Sebastian Wachsmann-Hogiu

Ian McConnell

A hand-held portable microarray reader for biodetection includes a microarray reader engineered to be small enough for portable applications. The invention includes a high-powered light-emitting diode that emits excitation light, an excitation filter positioned to receive the excitation light, a slide, a slide holder assembly for positioning the slide to receive the excitation light from the excitation filter, an emission filter positioned to receive the excitation light from the slide, a lens positioned to receive the excitation light from the emission filter, and a CCD camera positioned to receive the excitation light from the lens.

Hand-held portable microarray reader for biodetection

The present invention provides a novel method for producing recombinant baculoviruses expressing genes of interest by inserting a selectable marker gene, specifically the puromycin resistance (pac) gene, adjacent to the gene of interest into the baculovirus genome, and selecting for viruses that express the selectable marker. Selection of the recombinant virus in the presence of a selection condition, such as puromycin-containing cell culture media, simplifies virus production by eliminating the need for cloning, and testing the virus for gene expression. Such expression systems are readily adapted to an automated method for expression foreign genetic material in a high throughput manner.

Selection method for producing recombinant baculovirus

Growing community of inventors

Stockton, CA, United States of America

Joanna Albala