New England Biolabs, Inc.

Other

Joan E Brooks

Daniel F Heiter

Brian P Anton

Laura A Sznyter

Kimberly A Howard

Kimberly H O'Donnell

The present invention is directed to a method for cloning and producing the BglII endonuclease by: 1) introducing the restriction endonuclease gene from Bacillus globigii into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the BglII restriction endonuclease activity; and 3) purifying the BglII restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the BglII restriction endonuclease.

Method for cloning and producing the BglII restriction endonuclease and

A two-step method for cloning the DdeI restriction modification system is provided which comprises introducing the DdeI modification or methylase gene into a host cell and expressing the gene to protect the DdeI host cell followed by introducing the DdeI restriction or endonuclese gene into the host.

Method for producing the DdeI restriction endonuclease and methylase

A two-step method for cloning restriction modification systems is provided which comprises introducing the modification or methylase gene into a host cell and expressing the gene therein to protect the host cell followed by introducing the restriction or endonuclease gene into the host whereby the restriction gene is expressed at lower levels relative to the modification gene. Related methods for producing restriction enzymes is also disclosed as well as clones containing the BamI and DdeI restriction genes.

Method for cloning restriction modification system

A two-step method for cloning the BamHI restriction modification system is provided which comprises introducing the BamHI modification or methylase gene into a host cell and expressing the gene to protect the host cell followed by introducing the BamHI restriction or endonuclease gene into the host.

Method for producing the Bamhi restriction endonuclease and methylase

A device to provide a face lift without surgery comprising one member securely attached to a user's head and a second member slidingly attached to the first member with the second member have two ends each end being attachable to the face or other part of the head of the wearer with tension adjusting means as a part of the second member.

Instant face lift

The present invention is directed to a method for cloning and producing the Eag I restriction endonuclease by (1) introducing the restriction endonuclease gene from E. agglomerans ATCC into a host whereby the restriction gene is expressed; (2) fermenting the host which contains the vector encoding and expressing the Eag I restriction endonuclease, and (3) purifying the Eag I restriction endonuclease from the fermented host which contains the vector encoding and expressing the Eag I restriction endonuclease activity.

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Beverly, MA, United States of America

Joan E Brooks