Spatial Transcriptomics Ab

Illumina Corporation

10x Genomics, Inc.

10x Genomics Sweden Ab

Dynal As

Other

Cemu Bioteknik Ab

Spatial Transcriptomics Ab and Illumina, Inc.

Spatial Transcriptions Ab

Joakim Lundeberg

Jonas Frisen

Patrik Stahl

Alex Aravanis

Gordon M Cann

Leila Bazargan

Camilla Engblom

Jeffrey Mold

Mathias Uhlen

Patrik Ståhl

Fredrik Salmen

Kim Thrane

Qirong Lin

Rajiv Bharadwaj

Andrew D Price

Augusto Manuel Tentori

Zachary Bent

Yifeng Yin

Steven W Short

Felice Alessio Bava

Eswar Prasad Ramachandran Iyer

Cedric Uytingco

Jennifer Chew

David Maurice Cox

Linda Kvastad

Jonas Frisén

Mathias Uhl&eacute;n

Afshin Ahmadian

Meghan L F Frey

Fernandino Valdecanas

Fredrik SALMÉN

Patrik STÅHL

Sanja Vickovic

A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.

Imaging system hardware

Provided herein are methods, compositions, and kits for the detection of immune cell clonotypes and immune cell analytes within a biological sample.

Spatial transcriptomics for antigen-receptors

The present invention relates to methods and products for localized or spatial detection and/or analysis of RNA in a tissue sample or a portion thereof, comprising: (a) providing an object substrate on which at least one species of capture probe, comprising a capture domain, is directly or indirectly immobilized such that the probes are oriented to have a free 3' end to enable said probe to function as a reverse transcriptase (RT) primer; (b) contacting said substrate with a tissue sample and allowing RNA of the tissue sample to hybridise to the capture probes; (c) generating cDNA molecules from the captured RNA molecules using said capture probes as RT primers; (d) labelling the cDNA molecules generated in step (c), wherein said labelling step may be contemporaneous with, or subsequent to, said generating step; (e) detecting a signal from the labelled cDNA molecules; and optionally (f) imaging the tissue sample, wherein the tissue sample is imaged before or after step (c).

Methods and product for optimising localised or spatial detection of gene expression in a tissue sample

Localized detection of RNA in a tissue sample that includes cells is accomplished on an array. The array include a number of features on a substrate. Each feature includes a different capture probe immobilized such that the capture probe has a free 3' end. Each feature occupies a distinct position on the array and has an area of less than about 1 mm. Each capture probe is a nucleic acid molecule, which includes a positional domain including a nucleotide sequence unique to a particular feature, and a capture domain including a nucleotide sequence complementary to the RNA to be detected. The capture domain can be at a position 3&#x2032; of the positional domain.

Methods of detecting analytes

A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.

Spatially distinguished, multiplex nucleic acid analysis of biological specimens

Growing community of inventors

Lidingö, Sweden

Joakim Lundeberg