Leica Microsystems Cms Gmbh

Leica Microsystems Wetzlar Gmbh

Joachim Wesner

Michael Ganser

Albrecht Weiss

Gerhard Johannsen

A laser-micro-dissection method and a device for laser micro-dissection involves cutting a dissectate from a biological sample, which is applied to a planar carrier, by means of laser pulses along a closed cutting line. The parameters, which determine the laser pulses and the cut lines, are synchronous in relation to the laser pulses and are continually modified along the closed cut line. All elements which are arranged in the optical axis and which determine the parameters of the laser pulse and the cut lines, are controlled by a central calculation unit.

Laser-micro-dissection method and device for laser-micro-dissection

A method for blind deconvolution of microscopic images in which only a single parameter is varied for the estimation of the PSF. The single parameter takes account of the optical properties of the environment of the object () between the objective () of the microscope () and the region above the object. The single parameter represents a functional relationship among the parameters of the individual layers.

Method of guided blind deconvolution of microscopic images and software

A method and an apparatus for laser microdissection of specimen regions ( ) of interest of a specimen ( ) are described. In a first step, an incomplete cut line ( ) enclosing the specimen region ( ) of interest is generated by means of a laser beam ( ). At the incomplete point of the cut line ( ), there remains a web ( ) which joins the specimen region ( ) of interest to the surrounding specimen ( ). In a second step, the web ( ) is severed with a single laser pulse directed onto it, thereby completing the cut line. The specimen region ( ) of interest is in that context detached from the specimen ( ) and falls by the action of gravity into a collection vessel ( ).

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Lahnau, Germany

Joachim Wesner