Genequantum Healthcare (suzhou) Co., Ltd.

Lisure Science (suzhou) Co., Ltd

Boehringer Ingelheim Pharma Gmbh & Co Kg

Genequantum Healthcare Co., Ltd.

Joachim Walter

Gang Qin

Cao Lv

Sheng Zhou

Rolf Werner

Yunpeng Zhong

Gabriele Braunger

Jan Feuser

A conjugation device includes at least one flow reactor having an inlet and an outlet, the flow reactor(s) being completely filled with a support such as a matrix including 1) chromatography beads, fibers or membranes, and 2) a biologic catalyzer, namely the enzyme ligase, which is immobilized onto this support; a fluid delivery unit in fluid communication with the inlet of the flow reactor(s) and configured to continuously provide the flow reactor(s) with at least one kind of reaction fluid such as antibody and linker-payload according to stages of the conjugation process, the at least one kind of process fluid including a first moiety and a second moiety of a conjugate to be produced; and a fluid collection unit in fluid communication with the outlet of the flow reactor(s) and configured to control collection of fluid flowing out of the outlet of the flow reactor(s) according to the stages of the conjugation process. In a period of enabling the at least one kind of reaction fluid to continuously flow through the flow reactor(s), a conjugation reaction is conducted between the first moiety and the second moiety under catalysis of the ligase to produce the conjugate.

Conjugation device and method for producing conjugates

The present disclosure relates to the field of biotechnology. In particular, provided are a ligase fusion protein and an immobilized ligase comprising the same. Also provided is use of the ligase fusion protein or the immobilized ligase in the preparation of conjugates. Further provided is a process for the preparation of conjugates using a ligase or a ligase unit.

Ligase fusion proteins and application thereof

The present invention relates to the regeneration of adsorbent matrices that are used for the purification of substances from aqueous liquid samples derived from biological material. The method is adapted to adsorbent matrices that are sensitive to hydrolysis either because of a sensitive base matrix or a sensitive ligand including also a sensitive spacer and is characterized in contacting at least one regeneration solution with the adsorbent matrix which comprises an organic solvent which is water-miscible and has a pH value &#x2266;4, preferably &#x2266;3 (acid solution) or &#x2267;10 but &#x3c;13 (alkaline solution).

Method for regeneration of adsorbent matrices

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Mittelbiberach, Germany

Joachim Walter