Henkel Kommanditgesellschaft Auf Aktien

Henkel Kgaa

Henkel Research Corporation

Joachim Schindler

Rolf Schmid

Alfred Struve

Frank F Hill

Albrecht Weiss

Wolfgang Preuss

Friedhelm Bartnik

Michael Bahn

Karl Heinz Schmid

Ludwig Schieferstein

Adolf Asbeck

Christian G Paech

Rudolf Lehmann

Werner Stein

Charles R Wilson

Konrad Engelskirchen

Robert B Reynolds

Hans F Pfeiffer

Holger Viehweg

Dieter Hansen

Jonathan R Mielenz

Beth F Ladin

Martina Markgraf

Karlheinz Maurer

Wolfgang Schreiber

Heinrich Waldhoff

Nicholas C Kennedy

Sherman S Hom

A proteolytic enzyme for use in detergent formulations having increased pH and oxidative stability under typical laundering conditions in aqueous solutions is produced by fermenting Bacillus licheniformis host strain transformed by a multicopy plasmid comprised of DNA sequences which code for the desired proteolytic enzyme.

Alkaline proteolytic enzyme and method of production

A process for separation of multicomponent mixtures of dicarboxylic acids, especially, mixtures of C.sub.8 -C.sub.24 saturated and unsaturated acids is derived from a fermentation process, in which an aqueous feed solution containing the mixture of dicarboxylic acids is adjusted in pH value depending on the permeability of the component to be separated and over 95% of the saturated component is separated from the unsaturated material by passing the mixture is content with a membrane filter.

Process for the separation of dicarboxylic acids

A novel microorganism, mutant chol 149-K14 (DSM 2065) and process for the preparation of 3a.alpha.-H-4.alpha.-(3'-propionic acid)-5.alpha.-hydroxy-7a.beta.-methyl-hexahydro-1-indanone and the .delta.-lactone thereof comprising culturing a steroid compound of animal or plant origin with the above microorganism mutant in an aqueous nutrient medium under aerobic conditions.

Process and microorganism for degrading steroids

Troublesome foaming in the fermentation of yeast is suppressed by the addition of polyglycol ethers which are derived from saturated and monounsaturated C.sub.16 -C.sub.18 fatty alcohols and which contain on an average of from 1 to 4 ethylene and/or propylene glycol ether groups. Preferred defoamers contain oleyl and cetyl alcohol residues in a ratio of from 3:1 to 1:1, and on average 2 ethylene glycol ether groups. The defoamers do not inhibit yeast growth or lead to premature softening and liquefaction of the compressed yeast.

Defoamers for yeast fermentation

In the aerobic culture of microorganisms of the Xanthomonas genus in fermentation media, the use of water-in-oil emulsions in the media minimizes viscosity problems and enhances yields of Xanthomonas biopolymers. Preferably, the emulsion is formed with a surfactant and the microorganism is Xanthomonas campestris.

Process for producing Xanthomonas biopolymers

Process for the preparation of acid stable protease having a broad activity spectrum in high yields from mutants of the fungus Aspergillus, and the mutants used therein.

Acid stable protease from mutants of genus Aspergillus

Process for the preparation of acid stable protease having a broad activity spectrum by culturing selected mutants of Rhizopus rhizopodiformis CBS 227.75 under aerobic conditions in a nutrient medium containing assimilable carbon and nitrogen sources, at a pH between 3 and 7 and at a temperature between 25.degree. C. and 50.degree. C., and separating the resulting protease enzyme; the mutants used in the process together with a process for preparation of the mutants.

Acid stable protease from mutants of genus Rhizopus

This invention relates to a process for the preparation of a concentrate containing sterol compounds of natural origin, which concentrate is suitable as a starting material for the transformation of sterol compounds by fermentation, which comprises the steps of:

Transformation of sterols by fermentation

Sterol concentrates, the preparation thereof, and their use in the

For the production of 17-C-steroid-.alpha.-propionic acid compounds by microbial side chain degradation on 17-C-side chain steroid substrates wild strain microorganisms selected in predetermined manner are subjected to a multi-stage mutation and selection. The resultant mutant strains are capable of supplying the desired compounds, e.g. .DELTA.-4 BNC and/or .DELTA.-1,4 BNC in high yields even in the absence of inhibitors arresting the growth and/or degradation of the steroid ring. Wild strains and mutants particularly suitable, and their choice, production and use, are described.

Process for the preparation of defective mutants of microorganisms

A process for the production of useful steroids obtained by side chain degradation of sterol source materials through the use of microorganisms is described.

Steroid production

An acid stable protease whose pH range of 50% of maximum activity is between pH 2.5 and 6.5, produced by culturing a fungus strain of the species Rhizopus rhizopodiformis CBS 227.75 under aerobic conditions in a nutrient medium containing assimilable carbon and nitrogen sources, at a pH between 3 and 7 and at a temperature between 25.degree. C and 50.degree. C, and separating the enzyme produced; as well as the method of production, its use as a foodstuff additive and foodstuffs containing the same.

Growing community of inventors

Hilden, Germany

Joachim Schindler