Metanomics Gmbh

Metanomics Gmbh and Co. Kgaa

Max-planck-gesellschaft Zur F&#xf6;rderung Der Wissenschaften E. V.

Joachim Kopka

Ralf Looser

Tilmann B Walk

Lothar Willmitzer

Michael Manfred Herold

Martin Kluttig

Martin Dostler

Annette Hennig

Britta Lehmann

Achim Fegert

Silke Heidemann

Silke Klein

Alexander Lüdemann

Alexander Erban

Nicole Christiansen

Cornelia Wagner

Jörn Quedenau

Joachim Selbig

Alexander Ludemann

Ute Roessner-Tunali

Alisdair Fernie

Ewa Urbanczyk-Wochniak

Described is a method for analyzing the metabolites of a biological sample which comprises quantitatively determining one or more metabolites in said sample in a way that said quantitative determination resolves isotopic mass differences within one metabolite, said method being characterized in that the sample comprises or is derived from a cell which has been maintained under conditions allowing the uptake of an isotopically labeled metabolizable compound so that the metabolites in said cell are saturated with the isotope with which said metabolizable compound is labeled. This method may further comprise, prior to quantitative determining the metabolites, combining the biological sample (i.e. the first biological sample) with a second biological sample in which the metabolites are not isotopically labeled or are isotopically labeled differently from the first biological sample; and determining in said biological samples the relative quantity of metabolites which differ by their isotopical label. Furthermore described is a set of isotopically labeled metabolites obtainable by applying this method, as well as kits facilitating the application of this method and corresponding uses.

Method for analysing metabolites

The present invention relates to a process for extracting constituents from organic material, comprising the step of extracting with a monophasic mixture of 50% by volume to 90% by volume of methanol and 50 to 10% by volume of water and optionally 0% by volume to 40% by volume of a further solvent or solvent mixture.

Process for extracting constituents from organic material

The present invention relates to a process for isolating constituents from organic material, comprising the following process steps:

Method for extraction and analysis of contents made from organic material

Method for the extraction of components made from organic material

The invention relates to a method and device for analyzing a sample containing a number of substances. The method comprises the following steps: recording one or more mass spectra (mass spectra data D) of the sample (P); deconvoluting the mass spectra in a first evaluating device (A), which assigns the chromatographic peaks (a) and their associated deconvoluted mass spectra of one substance at a time based on the correspondence with a reference spectrum of the substance, and/or determining the intensity of the obtained peaks of the ions of the mass spectra in an evaluating device (B), which assigns the chromatographic peaks (b) of the ions and their associated mass spectra based on the correspondence of selective ions and their retention time ranges of the peaks (b) with the reference values of the substance predetermined for a substance, and verifying the assignments in a validating device (V).

Mass spectrometry analysis method and system

The invention relates to a method for the extraction of components made from organic material, comprising the step of extraction with a single-phase mixture of 50 to 90 vol. % methanol and 50 to 10 vol. % water and optionally 0 to 40 vol. % of a further solvent or solvent mixture.

Method for the extraction and analysis of contents made from organic material

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Berlin, Germany

Joachim Kopka