Novo Nordisk A/s

Jo Slashedrli Ringsted

Henrik Dalbo Slashedge

Thorkild Christensen

John Pedersen

Torben E Jessen

Process for preparing a desired protein

Amino-terminally extended human growth hormone (HGH)

Biosynthetic human growth hormone

A process for producing ripe human growth hormone is described which comprises introducing into bacteria a recombinant DNA vector comprising a DNA sequence encoding an amino-terminal extended human growth hormone which amino-terminal extension has a negatively charged amino acid sequence and has an even number of amino acids, growing the bacteria such that the amino-terminal extended human growth is expressed, separating the amino-terminal extended human grown hormone from contaminants, cleaving the amino-terminal extension with dipeptidyl aminopeptidase I to obtain ripe human growth hormone and isolating the ripe human growth hormone produced.

Growing community of inventors

Br.o slashed.ndby, Denmark

Jo Slashedrli Ringsted