Wisconsin Alumni Research Foundation

Jo E Handelsman

Eric V Stabb

Robert M Goodman

Sandra J Stewart

Amy Klimowicz

Jon C Clardy

Larry J Halverson

Changhui Guan

Lynn M Jacobson

David James Beebe

Duane S Juang

Jiaquan Yu

David W Johnson

Michelle Renee Rondon

Haiyin He

Laura Silo-Suh

Kevin P Smith

Jocelyn Louise Milner

Michael G Thomas

Bradley R Borlee

Philip J Balandyk

Doreen E Gillespie

Alan D Bettermann

Laura Silo

Yolande Alia Chan

Benjamin Lethbridge

Elizabeth Anne Blackson

Sean F Brady

Elizabeth A Stohl

Angela M Podevels

Lynn Wunderlich

Ellen H Mester

A platform and method for conducting multi-variable combinational interactions are provided. An array of multiplexing chambers in formed in a body. The body also includes a common well communicating with each multiplexing chamber of the array of multiplexing chambers and a plurality of variable wells. Each of variable wells communicates with at least one multiplexing chamber of the array of multiplexing chambers. The common well is loaded with a first variable and different variables are loaded in each of the plurality of variable wells. The interaction of the first variable with at least one of the different variables in each multiplexing chamber of the array of multiplexing chambers is observed.

Platform and method for multi-variable screening

Some HBL and NHE enterotoxins are known to cause food-borne diseases in humans. Enterotoxin-deficient mutants of member strains of thegroup that do not produce HBL, HBL, HBL, or NHE enterotoxins are disclosed. Enterotoxin-deficient mutants are suitable for use as biocontrol agents. Methods for making the mutants and for using the mutants are described.

Construction of a quadruple enterotoxin-deficient mutant of

Platform for multi-variable screening

Construction of a quadruple enterotoxin-deficient mutant of bacillus thuringiensis

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Novel extender units for Type I polyketide synthases are provided. Also provided are genes, compounds, and methods for generating these units, and for incorporation of the novel extender units into polyketides for the purpose of generating new structural derivatives of polyketide-containing products.

Type I polyketide synthase extender units

The present invention provides compositions and methods for modulating bacterial quorum sensing using antagonist or agonist compounds. Further, the present invention provides methods of treating or preventing microbial damages and diseases, in particular for diseases where there is an advantage in inhibiting quorum sensing regulated phenotypes of pathogens.

Method for modulating microbial quorum sensing

Disclosed are triaryl cationic compounds that exhibit broad spectrum antibiotic and antifungal activity, pharmaceutical compositions containing the compounds, and methods of treating bacterial and fungal infections using the compounds. The compounds were initially isolated by screening a 25,000-member bacterial artificial chromosome (BAC) library of environmental (eDNA) from soil. At least one clone produced a dark brown melanin-like compound that was found to have antibiotic activity. The compounds were isolated and synthesized de novo. From within the positive clone, a single open reading frame that shares extensive sequence similarity with members of the 4-hydroxyphenylpyruvate family of enzymes was found to be necessary and sufficient to confer the production of at least one of the subject compounds on

Triaryl cation antibiotics from environmental DNA

The present invention provides methods and composition for accessing, in a generally unbaised manner, a diverse genetic pool for genes involved in biosynthetic pathways. The invention also provides compounds which can be identified by cloning biosynthetic pathways.

Microorganism genomics, compositions and methods related thereto

A enterotoxin-deficient mutant of a member strain of the group does not produce HBL enterotoxin, which has been regarded as a human pathogen found in member strains. An enterotoxin-deficient mutant is suitable for use as a biocontrol agent. Methods for making the mutant and for using the mutant are described.

Enterotoxin-deficient bacillus

The present invention provides methods and compositions for accessing, in a generally unbaised manner, a diverse genetic pool for genes involved in biosynthetic pathways. The invention also provides compounds which can be identified by cloning biosynthetic pathways.

A novel strain of Bacillus cereus, designated W35 is useful as a biocontrol agent to combat fungal damping off disease in field crop plants. W35 produces the antibiotic zwittermicin A. Strain W35 is distinguished from other zwittermicin-producing Bacillus cereus strains by resistance to phage P7, antibiotic resistance, and colony morphology.

Bacillus cereus strain W35

A biocontrol bacteria, Bacillus cereus ATCC 53522, has been found to naturally synthesize two antibiotics, here designated zwittermicin A and antibiotic B. Zwittermicin A is a 396 dalton linear aminopolyol, a class of metabolite previously unknown in B. cereus. Antibiotic B is an aminoglycoside. Both antibiotics exhibit inhibitory activity on many fungal and bacterial pathogens.

Fungicidal toxins from biocontrol bacteria

A novel strain of Bacillus cereus, designated Z8 is useful as a biocontrol agent to combat fungal damping off disease in field crop plants. Z8 produces the antibiotic zwittermicin A. Strain Z8 is distinguished from other zwittermicin-producing Bacillus cereus strains by resistance to phage P7, antibiotic resistance, and colony morphology.

Bacillus cereus strain Z8

A novel strain of Bacillus cereus, designated SOY130 ATCC 202076 is useful as a biocontrol agent to combat fungal damping off disease in field crop plants. SOY130 produces the antibiotic zwittermicin A. Strain SOY130 is distinguished from other zwittermicin-producing Bacillus cereus strains by resistance to streptomycin and neomycin.

Growing community of inventors

Madison, WI, United States of America

Jo E Handelsman