Roche Sequencing Solutions, Inc.

Kapa Biosystems, Inc.

Jo-Anne Elizabeth Penkler

Brian Christopher Godwin

Joseph Platzer

Bronwen Miller

Janine Mok

Martin Ranik

Eric Van Der Walt

Daniel Burgess

Alexander Lovejoy

Improved methods and compositions are provided herein for primer extension target enrichment of target polynucleotides.

Primer extension target enrichment

The present disclosure provides a method for enrichment of at least one target nucleic acid in a library of nucleic acids. A first oligonucleotide is hybridized to a target nucleic acid in library of nucleic acids having first and second adapters. The hybridized first oligonucleotide is extended with a first polymerase, thereby producing a first primer extension complex including the target nucleic acid and the extended first oligonucleotide. The first primer extension complex is captured, enriched relative to the library of nucleic acids, and a second oligonucleotide is hybridized to the target nucleic acid. The hybridized second oligonucleotide is extended with a second polymerase, thereby producing a second primer extension complex including the target nucleic acid and the extended second oligonucleotide, and further liberating the extended first oligonucleotide from the first primer extension complex.

Target enrichment by unidirectional dual probe primer extension

Growing community of inventors

Cape Town, South Africa

Jo-Anne Elizabeth Penkler