Academia Sinica

Other

Jiun-Bo Chen

Tse Wen Chang

Pheidias C Wu

Alfur F Hung

Tse-Wen Chang

Chien-Jen Lin

Nien-Yi Chen

Tsewen Chang

Yu-Yu Shiung

A novel humanized anti-IgE antibody is disclosed. The antibody is capable of binding to free IgE, membrane-bound IgE on B lymphocytes, IgE bound by CD23, but not to IgE bound by high-affinity IgE.Fc receptor on mast cells. The present invention relates to the treatment of IgE-mediated diseases, including allergic asthma, allergic rhinitis, atopic dermatitis, food allergy, chronic spontaneous (idiopathic) urticaria, chronic rhinosinusitis, systemic mastocytosis, cutaneous mastocytosis, allergic bronchopulmonary aspergillosis, recurrent idiopathic angioedema, and eosinophil-associated gastrointestinal disorder by administering the anti-IgE antibody of the present invention.

Humanized anti-IgE antibodies that crosslink CD23 on B lymphocytes but do not sensitize mast cells

Antibodies binding to junction regions between the CH4 and C&#x3b5;mX domains of membrane-bound IgE and uses thereof in treating IgE-mediated diseases such as allergic diseases.

Anti-mIgE antibodies that bind to the junction between CH4 and C&#x3b5;mX domains

The invention pertains to methods of using C&#x3b5;mX peptides (e.g., GLAGGSAQSQRAPDRVL; SEQ ID NO:2) that can bind effectively induce immune responses to membrane-bound IgE (mIgE) expressed on the surface of human B lymphocytes.

C(epsilon)mX peptides for inducing immune responses to human mIgE on B lymphocytes

The invention pertains to the generation and utility of antibodies that can bind effectively to C&#x3b5;mX domain on membrane-bound IgE (mIgE) expressed on the surface of human B lymphocytes. The C&#x3b5;mX domain of 52 amino acid residues, located between the CH4 domain and the C-terminal membrane-anchor peptide on human membrane-bound epsilon chain, had been suggested as an antigenic site for immunological targeting of B cells expressing mIgE. Previous reported monoclonal antibodies, including a20, which bind to RADWPGPP (SEQ ID NO:1) peptide at the C-terminal of C&#x3b5;mX, have now been found to bind poorly to mIgE on human B cells. We have discovered that only monoclonal antibodies specific for certain segments, such as GLAGGSAQSQRAPDRVL (SEQ ID NO:2) and HSGQQQGLPRAAGGSVPHPR (SEQ ID NO:3), of C&#x3b5;mX can bind effectively to mIgE on human B cells and hence have the utility for targeting those B cells for the treatment of diseases mediated by IgE.

Growing community of inventors

Taipei, Taiwan

Jiun-Bo Chen