Indian Oil Corporation Limited

Department of Biotechnology

Jitendra Kumar Saini

Sankara Sri Venkata Ramakumar

Suresh Kumar Puri

Deepak Kumar Tuli

Ravi Prakash Gupta

Anshu Shankar Mathur

Simranjeet Kaur Sandhu

Mukund Adsul

Reeta Rani Singhania

The present invention relates to a mutant fungal strain of'MRJ-16' characterized by the ability to produce high titer of enzyme mixture comprising FPase, CMCase, Cellobiase, &#x3b2;-glucosidase, endoglucanase, &#x3b1;-L arabinofuranosidase, &#x3b2;-xylosidase, xylanase, pectinase, cellobiohydrase and oxidases and produce enzymes in the presence of a catabolite repressor molecule like glucose and/or xylose. The titer of enzyme mixture produced using mutant fungal strain MRJ-16 is at least two fold higher than naivestrain NCIM 1228, when used in a fermentation process. The mutant strain 'MRJ-16' with high hydrolytic activity and catabolite derepressed character is having application in the method of degrading or saccharifying biomass to produce valuable products for example-bioethanol.

Method for preparation and screening of fungal mutant with high hydrolytic activity and catabolite derepressed character

Growing community of inventors

Faridabad, India

Jitendra Kumar Saini