United States of America, As Represented by the Secretary of Agriculture

Other

University of Kentucky Research Foundation

Jitender P Dubey

Dante S Zarlenga

Cathleen Coss

Dolores Hill

Mark C Jenkins

Clara K Fenger

David E Granstrom

Nicola C Lally

Luis H Jauregui

Alvin A Gajadhar

Joan K Lunney

James Higgins

Recombinant proteins have been developed for the detection ofoocyst proteins for example in biological fluids. Isolated DNA sequences which encode these proteins have also been developed. The DNA sequences may be inserted into recombinant DNA molecules such as cloning vectors or expression vectors for the transformation of cells and the production of the proteins.

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Oligonucleotide primer sequences have been designed which are capable of uniquely amplifying DNA from when utilized in a polymerase chain reaction assay. In addition, a probe effective for detection of amplification products, particularly in a fluorogenic real time assay system has also been designed. The primers and probe are derived from the internal transcribed spacer 1 (ITS1) region of the 18S rRNA gene.

Polymerase chain reaction assay for the detection of Toxoplasma gondii

Antigens for the detection of antibodies to Neospora parasites for the diagnosis of neosporosis have been identified. Recombinant antigens may be produced by expression of DNA sequences derived from Both antigens are capable of detecting antibody responses in animals experimentally inoculated with but show no evidence of cross-reactivity with serum from animals inoculated with closely related parasites such as or Sarcocystis species.

Recombinant antigens useful for the serodiagnosis of neosporosis

An amplification primer and probe which can be used in an in vitro diagnostic test for the presence of S. neurona in equine blood or cerebrospinal fluid. Sarcocystis neurona is responsible for the equine condition of protozoal myelitis. The amplification primer is seventeen nucleotides in length and complementary to a unique section of the small ribosomal subunit of Sarcocystis neurona. The primer encompasses nucleotide positions 1470-1487 of the small ribosomal subunit of S. neurona. The primer has the sequence 5' CCATTCCGGACGCGGGT SEQ ID NO:1.

Sarcocystis neuronadiagnostic primer and its use in methods of equine

Growing community of inventors

Greenbelt, MD, United States of America

Jitender P Dubey