Kikkoman Shoyu Co., Ltd.

Jiro Ishiyama

Tamotsu Yokotsuka

Fumihiko Yoshida

Motohiko Kato

Yoshie Motomura

Nobuyuki Yamaji

A composition for use in the treatment for developing seedless fleshy berry of grapes. By treating the flower bunches of a grape tree with a composition containing gibberellin and cyclic 3',5'-adenylic acid in the form of an aqueous solution, it became possible to make seedless fleshy berry from grape trees belonging to varieties other than Delaware, namely belonging to Campbell-Arley, Berry A, Niagara, Kyoho, etc., from which seedless fleshy berry cannot be made by the conventional treatment with gibberellin.

Process for developing seedless fleshy berry of grapes

A process for producing 3',5'-cyclic adenylic acid in a high yield at a lower price by culturing a strain belonging to the genus selected from the group consisting of Corynebacterium, Arthrobacter and Microbacterium such as, for example, Corynebacterium murisepticum No. 7-10 (ATCC 21977), Arthrobacter 11-211 (ATCC 21978) Microbacterium No. 205-CM7 (ATCC 21979), Microbacterium No. 205-CM-XA3 (ATCC 21980), or Microbacterium No. 205-MP-197 (ATCC 21976) in a medium containing nutrient sources such as, carbon sources, nitrogen sources, inorganic salts and the like but containing no precursors and having a pH of 5-9 at a temperature of 20.degree.-40.degree. C under aerobic condition. The obtained 3',5'-cyclic adenylic acid is well-known for its participation in various biochemical reactions in vivo and for its active role as a mediator to various hormones. It has therefore always been highly evaluated biochemical reagent.

Process for producing 3',5'-cyclic adenylic acid

3' ,5'-Cyclic-adenylic acid or 3' ,5'-cyclic-deoxadenylic acid can be obtained in a high purity and a high yield by adding a water-soluble organic solvent such as methyl alcohol, ethyl alcohol, n-propyl alcohol, isopropyl alcohol, tert-butyl alcohol, acetone, N,N'-dimethylformamide, dioxane, tetrahydrafuran, ethyleneglycolmonomethylether and the like singly or in combination to an aqueous solution containing as impurities one or more kinds of nucleotides other than 3' ,5'-cyclicadenylic acid and 3' ,5'-cyclic-deoxyadenylic acid, nucleosides, nucleobases, colored substances, proteins and the like in addition to 3' ,5'-cyclic-adenylic acid or 3', 5'-cyclic-deoxyadenylic acid. The said 3' ,5'-cyclic-adenylic acid or 3' ,5'-cyclic-deoxyadenylic acid are useful and valuable as a biochemical reagent.

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Noda, Japan

Jiro Ishiyama