Pioneer Hi-bred International, Inc.

Her Majesty the Queen in Right of Canada, As Represented by the Minister of Agriculture and Agri-food

Her Majesty the Queen in Right of Canada As Represented by the Ministerof Agriculture and Agri-food Canada

Plant Research International B.v.

Jiro Hattori

Brian Miki

Eric Barbour

Chris L Baszczynski

Teresa Martin-Heller

Keqiang Wu

Elizabeth Foster

Lining Tian

Peijun Zhang

Kamal Malik

Th&eacute;r&egrave;se Ouellet

Thérèse Ouellet

Hélène Labbé

Daniel Charles William Brown

Kim Boutilier

Jan Custers

Michiel Van Lookeren Campagne

Daniel C Brown

Raymond Tropiano

David A Theilmann

T-DNA tagging with a promoterless &#x3b2;-glucuronidase (GUS) gene generated transgenicplants that expressed GUS activity either only in developing seed coats, or constitutively. Cloning and deletion analysis of the GUS fusion revealed that the promoter responsible for seed coat specificity was located in the plant DNA proximal to the GUS gene. Analysis of the region demonstrated that the seed coat-specificity of GUS expression in this transgenic plant resulted from T-DNA insertion next to a cryptic promoter. This promoter is useful in controlling the expression of genes to the developing seed coat in plant seeds. Similarly, cloning and characterization of the cryptic constitutive promoter revealed the occurrence of several cryptic regulatory regions. These regions include promoter, negative regulatory elements, transcriptional enhancers, core promoter regions, and translational enhancers and other regulatory elements.

Cryptic regulatory elements obtained from plants

The present invention provides for a gene obtained during the induction of microspore embryogenisis. The protein encoded by this gene renders plant cells embryongenic, and increases the regenerative capacity of the plant cell. Also disclosed is the regulatory region of this gene and its use for directing the expression of a gene of interest within a suitable host cell.

Use of the BNM3 transcriptional activator to control plant embryogenesis and regeneration processes

The present invention is directed to translational regulatory elements that mediate the amount of protein produced within a host capable of expressing a construct comprising one or more translational regulatory elements in operative association with a gene of interest. These translational regulatory elements were derived from T1275 (tCUP) and exhibit a high degree of similarity with members of the RENT family of repetitive elements. Translational regulatory elements are disclosed that either increase or decrease he amount of protein produced within the host organism. These translational elements are operative in a wide range of hosts including plant, animals, yeast, fungi and bacteria. Analogs, derivatives and fragments of these translational elements are also disclosed.

Translational regulatory elements

A novel DNA regulatory element that confers microspore-specific gene expression has been discovered, isolated, and characterized. The microspore-specific regulatory element can be used to control the expression of a foreign gene that disrupts the function of microspores. Thus, the control of pollen production can be achieved by using the microspore-specific regulatory element to produce male-sterile plants. Various methods can be used to restore male fertility in the F1 generation of such male-sterile plants. In addition, the microspore-specific regulatory element can be used to confer resistance to viral and insect pests.

Use of a microspore-specific regulatory element for production of virus

Microspore-specific regulatory element

Growing community of inventors

Ottawa, Canada

Jiro Hattori