Ustav Organicke Chemie a Biochemie Av Cr, V.v.i.

Ustav Makromolekularni Chemie Av Cr, V.v.i.

Ustav Organicke Chemie a Biochemie Akademie Ved Cr, V.v.i.

Univerzita Karlova V Praze, Prirodovedecka Fakulta

Univerzita Karlova

Jiri Schimer

Jan Konvalinka

Pavel Sacha

Pavel Majer

Karel Ulbrich

Jiri Strohalm

Vladimir Subr

Vaclav Navratil

Tomas Knedlik

Petr Cigler

Jan Tykvart

Frantisek Sedlak

A method for detection of active form of analytes in a sample and/or for determination of ability of tested substances to bind to the active site of these analytes has the following steps: a) analyte or group of analytes from the sample is immobilized on the surface of a solid carrier; b) analyte or group of analytes is incubated with a detection probe; c) then the solid carrier is washed to remove unbound detection probe; and subsequently, the amount of bound detection probe is determined.

Method of detection of analyte active forms and determination of the ability of substances to bind into analyte active sites

A macromolecular water-soluble conjugates based on synthetic copolymers to which at least one affinity tag, at least one imaging probe and at least one targeting ligand are bound via covalent bonds. The macromolecular conjugate may be used in identification, visualization, quantification or isolation of proteins and/or cells.

Macromolecular conjugates for visualization and separation of proteins and cells

Synthetic macromolecular conjugate for selective interaction with proteins has a synthetic copolymer, and at least one binding group and at least one further group selected from an affinity tag and an imaging probe, and at least one binding group and at least one further group being bound via covalent bond to the synthetic copolymer. The macromolecular conjugate is suitable in particular for identification, visualization, quantification or isolation of proteins and/or cells.

Macromolecular conjugates for isolation, immobilization and visualization of proteins

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Prague, Czechia

Jiri Schimer