Ceskoslovenska Akademie Ved

Jiri Lenfeld

Jiri Coupek

Frantisek Svec

Jan Peska

Milan Benes

Zedenek Plichta

The invention pertains to a method for the production of bead dextran materials for gel chromatography. The subject of the invention is a method for the production of bead dextran materials for gel chromatography, wherein dextran is, before or during the suspension process or in both stages, subjected to reduction in an alkaline solution, advantageously by the action of sodium borohydride, whereas the density of crosslinking is controlled above all by variation of the concentration of the initial dextran solution and the resulting gel may be optionally subjected to the additional crosslinking in a separate subsequent operation. The procedure according to the invention provides the product of higher quality with a lower content of carboxylic groups and substantially facilitates the preparation of gels with a very high and a very low crosslink density.

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Prague, Czechia

Jiri Lenfeld