Other

Wuhan Grand Hoyo Co., Ltd.

Jiong Wang

Jing Li

Kun Wan

Xuechen Mei

Mengjun Song

Panpan Xing

Haixia Su

Aifu Liu

Provided is a method for screening aconstitutive expression vector promoter on the basis of transcriptome sequencing; and further provided are theconstitutive expression vector promoter screened on the basis of transcriptome sequencing, an expression vector comprising the promoter, a recombination strain obtained by transforming a host cellusing the expression vector, and applications thereof.

constitutive expression vector promoter screened on the basis of transcriptome sequencing, screening method thereof, and applications thereof

Disclosed is a L-isoleucine-producingfermentation medium, comprising a basal medium and a growth factor, wherein the growth factor consists of choline, betaine and vitamin B6, and the contents of each ingredient in the fermentation medium are: 0.2-1 g/L choline, 0.25-0.5 mg/L betaine, and 0.05-0.3 mg/L vitamin B6. Also disclosed is a method for cultivating the L-isoleucine-producing, comprising: inoculating the L-isoleucine-producingonto the fermentation medium, wherein the volume of the bacteria liquid accounts for 5-20% of the volume of the fermentation medium, adjusting the pH to 6.5-7 with aqueous ammonia, controlling the dissolved oxygen to 30-50%, and fermenting for 25-30 h; then decreasing the dissolved oxygen to 15-25%, and feeding a 50-80% glucose solution into the fermentation broth to control the residual sugar at 3-4%, continuing the fermentation until 60-70 hours, then terminating the fermentation, and controlling the temperature of the overall fermentation process at 29-33&#xb0; C.

L-isoleucine-producing corynebacterium glutamicum fermentation medium and culture method

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Wuhan, China

Jiong Wang