Chinese Academy of Sciences

Jiong Li

Kexiao Zheng

Min Jiang

Kefeng Pu

Chao Chen

A screening method is provided. Cells secreting target antibodies are screened by mixing candidate cells labeled with a first fluorescent molecule, a capture antigen and a labeled antibody against a target antibody and incubating, labeling using a high content cell imager and sorting using flow cytometry so as to screen cells secreting target antibodies. The screening method disclosed in the present application can automatically complete the labeling and sorting of target candidate cells in high throughput by labeling with a fluorescent molecule in combination with high-content cell imager and flow cytometer, so as to provide sufficient quantity of cells for subsequent amplification to obtain their antibody sequences and screen affinity antibodies. This method greatly improves the screening efficiency.

Screening method

The present application discloses a magnetic microchip having a graph code as well as a preparation method and application thereof. The magnetic microchip comprises: a graph code comprising more than one opaque microstructure mainly consisting of colloid aggregates formed by aggregating magnetic solid particles, wherein adjacent magnetic solid particles are isolated at least by an organic molecule and/or an inorganic molecule; and a transparent encapsulating structure at least used for wrapping the opaque microstructure. The magnetic microchip of the present application is prepared by adopting a micromachining process and has high in machining precision and repeatability, the size difference between different batches or between different individuals at the same batch can be ignored, use is convenient, and accuracy of a detection result can be effectively ensured, thereby greatly improving analysis quality.

Magnetic microchip having graph code as well as preparation method and application thereof

A micro-liquid phase reaction method based on a substrate with a hydrophilic-hydrophobic patterned surface, including the following: applying a liquid phase system containing a hydrotropic substance and/or an amphipathic substance to a hydrophobic smooth plane in a sample-spotting manner to form an array of tiny droplets, subsequently removing the solvent in each droplet to bond the hydrotropic substance and/or amphipathic substance in each droplet to the hydrophobic smooth plane so as to form an array of hydrophilic bonding points, then moving an aqueous phase system or hydrophilic liquid phase system containing more than one reactants over the hydrophobic smooth plane, thereby forming island-like tiny reaction droplets at each hydrophilic bonding point, and finally under the set reaction conditions, reacting the reactants in each tiny reaction droplet. The method allows a parallel processing system for multiple reactions to be implemented under common experiment conditions, and greatly extends the application range thereof.

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Suzhou, China

Jiong Li