University of Pennsylvania

Jinzhao Song

Haim H Bau

Changchun Liu

Michael G Mauk

Frederic Bushman

Scott Sherrill-Mix

Karen E Ocwieja

Disclosed herein are methods for multiple stage isothermal amplification of nucleic acid comprising a first substantially isothermal amplification reaction on the nucleic acid to generate a first amplification product and at least one substantially isothermal amplification reaction on the first amplification product to generate at least one second amplification product in an amount sufficient for recovery, testing, or characterization.

Multiple stage isothermal enzymatic amplification

Provided herein include compositions comprising a primer having the nucleic acid sequence of ACeIN-F3_c, a primer having the nucleic acid sequence of ACeIN-B3_a, a primer having the nucleic acid sequence of ACeIN-B3_b, a primer having the nucleic acid sequence of ACeIN-FIP_e, a primer having the nucleic acid sequence of ACeIN-FIP_f, a primer having the nucleic acid sequence of ACeIN-BIP (or ACeIN-BIP-song), a primer having the nucleic acid sequence of ACeIN-LF; and a primer having the nucleic acid sequence of ACeIN-LB. Also provided are methods of detecting human immunodeficiency virus (HIV) nucleic acids in a sample comprising performing reverse transcription-based loop mediated isothermal amplification (RT-LAMP) on a sample using the previously disclosed compositions.

Compositions, kits, and methods to detect HIV virus

Growing community of inventors

Philadelphia, PA, United States of America

Jinzhao Song