Lg Life Sciences, Limited

Lg Chem, Ltd

Jinseok Kang

In Soo Kim

Gihune Jung

Hee Ju Kim

Young Suk Park

Young Mee Lee

Ji Hyun Kwon

Young Soo Seo

Provided is a method of manufacturing a plastic substrate with lower auto-fluorescence and better specificity. The method includes: (a) preparing a plastic substrate having an Atomic Force Microscopic (AFM) surface roughness of R&#x3c;3 nm or R&#x3c;4 nm under the condition of 50 &#x3bc;m&#xd7;50 &#x3bc;m or less; (b) treating the plastic substrate with plasma; and (c) treating the plastic substrate with a surface-modifying monomer. A plastic substrate manufactured by the method is also provided. The plastic substrate exhibits a remarkably low auto-fluorescence and thus better specificity for detection of target biomolecules, which enables the broad application of a plastic substrate, which can be easily designed to include a microfluidic structure relative to a glass substrate but has been limitedly used due to high auto-fluorescence, to microarrays, biochips, or well plates. Furthermore, the method of manufacturing the plastic substrate enables an enhancement in the surface specificity of the plastic substrate and easy adjustment of the surface characteristics of the plastic substrate.

Method of manufacturing plastic substrate using plasma process and plastic substrate manufactured using the method

Provided are a PNA chip for determining the genotypes of mycobacterial species in which probe PNAs having DNA sequences of SEQ ID NOs: 5-28 are immobilized on a plastic substrate coated with an epoxy group-containing polymer, and a method of determining the genotypes of mycobacterial species using the PNA chip. According to the PNA chip and the method of determining the genotypes of mycobacterial species, the genotypes of mycobacterial species which are causes of tuberculosis and respiratory diseases can be determined within a short term with good sensitivity and specificity. Therefore, detection of the presence of mycobacterial species in test samples, and after confirmation of the presence of mycobacterial species, identification of the mycobacterial species and determination of whether or not the mycobacterial species are tuberculosis-causing mycobacterial species can be achieved promptly and accurately.

PNA chip for determining genotypes of mycobacterial species using plastic substrate coated with epoxy group-containing polymer and method of determining genotypes of mycobacterial species using the PNA chip

Growing community of inventors

Suwon, South Korea

Jinseok Kang