Pioneer Hi-bred International, Inc.

E.i. Dupont De Nemours and Company

Other

Jinrui Shi

Pramod B Mahajan

Hongyu Wang

Bailin Li

Shane E Abbitt

George William Singletary

Huirong Gao

David S Ertl

Matthias Liebergesell

Marianna Faller

Kathleen Schellin

Rebecca E Cahoon

Stephen M Allen

Saverio Carl Falco

William James Gordon-Kamm

J Antoni Rafalski

Anthony John Kinney

Keith S Lowe

Andrew Mark Cigan

Zhongsen Li

Timothy George Helentjaris

Hajime Sakai

Omolayo O Famodu

Benjamin A Bowen

Perry G Caimi

Guihua Lu

Christopher L Baszczynski

Guo-Hua Miao

Emil M Orozco, Jr

Joshua K Young

Yiwen Fang

Zhan-Bin Liu

Jeffrey Habben

Larry R Beach

Zude Weng

Sergei Svitashev

John A McElver

Jian-Ming Lee

Chun Ping Li

Zhan-bin Liu

Jennie Bih-Jien Shen

Susan J Martino-Catt

Kathryn K Lappegard

Jan Antoni Rafalski

Shawn L Anderson

Alan R Rendina

Karin N Lohman

L Aleksander Lyznik

Sabrina Humbert

Ilham L Zoughi

Heidi G Major Sleister

Yunsheng Wu

Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.

Plant genome modification using guide RNA/Cas endonuclease systems and methods of use

The disclosure provides plant, plant parts, plant cells, seed and grain containing a targeted genetic modification that inserts an inverted repeat sequence into a gene. The disclosure further provides hybrid plants that contain the inverted repeat sequence in a single allele of the gene. Further provided are methods for decreasing expression of a gene encoding a polypeptide of interest in a hybrid plant by crossing a plant comprising the targeted genetic modification with a plant not comprising the genetic modification to produce a hybrid plant that contains the inverted repeat sequence in a single allele of the gene.

Dominant CRISPR inverted-repeat alleles to down-regulate gene expression in heterozygous plants

The present invention provides compositions and methods for regulating expression of nucleotide sequences of interest in a plant. Compositions are novel nucleotide sequences for a seed-preferred promoter associated with the maize 17 KD OLE (17 kilodalton oleosin) coding region. A method for expressing a nucleotide sequence of interest in a plant using the regulatory sequence disclosed herein is provided.

Maize 17KD oleosin seed-preferred regulatory element

Compositions and methods are provided for modulating the level of phytate in plants. More specifically, the invention relates to methods of modulating the level of phytate utilizing nucleic acids comprising multidrug resistance-associated protein (MRP) nucleotide sequences to modulate the expression of MRP(s) in a plant of interest. The compositions and methods of the invention find use in agriculture for improving the nutritional quality of food and feed by reducing the levels of phytate and/or increasing the levels of non-phytate phosphorus in food and feed. The invention also finds use in reducing the environmental impact of animal waste.

Maize multidrug resistance-associated protein polynucleotides and methods of use

Compositions and methods for modulating MIOX activity are provided. Such compositions include nucleotide sequences for novel MIOX sequences obtained from maize, amino acid sequences for the proteins encoded by the nucleotide sequences of the invention, and variants and fragments thereof. Methods of the invention involve introducing into a plant a nucleotide construct comprising a MIOX nucleotide sequence operably linked to a promoter that drives expression in a plant. Expression of the novel nucleotide sequences disclosed herein confers advantageous agronomic properties on a plant. Transformed plants, plant cells, and seeds are additionally provided.

Modulating myo-inositol catabolism in plants

Compositions and methods are provided for modulating the level of phytate in plants. More specifically, the invention relates to methods of modulating the level of phytate utilizing nucleic acids comprising Ins (1,3,4,5,6)P2-kinase (IP2K) nucleotide sequences to modulate the expression of IP2K in a plant of interest. The compositions and methods of the invention find use in agriculture for improving the nutritional quality of food and feed by reducing the levels of phytate and/or increasing the levels of non-phytate phosphorus in food and feed. The invention also finds use in reducing the environmental impact of animal waste.

Phytate polynucleotides and methods of use

This invention relates to newly identified polynucleotides and polypeptides in the phytic acid biosynthetic pathway, variants and derivatives of same; methods for making the polynucleotides, polypeptides, variants, derivatives and antagonists. In particular the invention relates to polynucleotides and polypeptides of the inositol polyphosphate kinase gene family. In particular this invention relates to using the newly identified polynucleotides and polypeptides to modulate phytic acid biosynthesis in such a way as to decrease phytate and/or increase non-phytate phosphorous in plants.

Inositol polyphosphate kinase genes and uses thereof

This invention relates to newly identified polynucleotides and polypeptides in the phytic acid biosynthetic pathway, fragments, variants and derivatives of same; methods for making the polynucleotides, polypeptides, fragments, variants, derivatives and antagonists. In particular the invention relates to polynucleotides and polypeptides of the inositol 1,3,4-trisphosphate 5/6-kinase gene family. In particular this invention relates to using the newly identified polynucleotides and polypeptides to modulate the phytic acid biosynthesis in such a way as to decrease phytate and/or increase non-phytate phosphorous, especially in corn or soy animal feedstuffs.

[object Object]

The present invention provides compositions and methods for regulating expression of isolated nucleotide sequences in a plant. The compositions are novel nucleic acid sequences for seed-preferred regulatory sequences. Methods for expressing an isolated nucleotide sequence in a plant using the regulatory sequences are also provided. The methods comprise transforming a plant cell to contain an isolated nucleotide sequence operably linked to the seed-preferred regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.

Seed-preferred regulatory elements and uses thereof

This invention relates to newly identified polynucleotides and polypeptides in the phytic acid biosynthetic pathway, variants and derivatives of same; methods for making the polynucleotides, polypeptides, variants, derivatives and antagonists. In particular the invention relates to polynucleotides and polypeptides of the inositol polyphosphate kinase gene family. In particular this invention relates to using the newly identified polynucleotides and polypeptides to modulate the phytic acid biosynthesis in such a way as to decrease phytate and/or increase non-phytate phosphorous, especially in corn or soy animal feedstuffs.

The invention provides isolated RAD51C nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering RAD51C levels in plants. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions.

RAD51 polypeptides and uses thereof

The invention provides isolated polynucleotides encoding MRE11 binding polypeptides, recombinant expression cassettes, host cells, and transgenic plants comprising said polynucleotides.

Polynucleotide encoding MRE11 binding polypeptide and uses thereof

The invention provides an isolated RAD51C nucleic acid from maize. The present invention provides methods and compositions relating to altering RAD51C levels in plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants.

RAD51-like polynucleotide and uses thereof

Growing community of inventors

Johnston, IA, United States of America

Jinrui Shi