Biochain Institute, Inc.

Jinming Xia

Xiaoliang Han

Zhongdong Liu

This invention presents the high throughput (HT) analysis technology for molecular fractions employing micro-array format. Molecules are fractionated according to the properties of molecules and are assigned primary designated orders while their tissue origins are assigned secondary designated orders such that each fractionated molecules become addressable and traceable. Fractionated molecules are recovered, arrayed, and analyzed according to the same primary designated orders and secondary designated order in a high throughput manner. The above properties of molecules can be measured and predicted.

High throughput analysis for molecular fractions

This invention presents a set of methods to extract DNA, RNA and protein simultaneously from biomaterials by reagents with high pH. DNA and RNA can be extracted from a upper aqueous phase simultaneously either together as a DNA and RNA mixture or separated DNA and RNA. Protein can be extracted from lower organic phase. The DNA and RNA mixture can be used either as DNA or RNA directly depending on applications without further separation, or as resource for the separated DNA and RNA that can be selected from the DNA and RNA mixture by selective precipitation and/or by selective enzyme digestions. This invention provides the choice of extraction either of DNA and RNA mixture or of separated DNA and RNA simultaneously, as well as extraction of protein from the same piece of biomaterials in high quality, which is very critical for biomaterials with limited resource, such as clinical specimens.

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San Francisco, CA, United States of America

Jinming Xia