Beijing Xiaomi Mobile Software Co., Ltd.

Academy of Military Medical Science, Pla

Institute of Biotechnology, Academy of Military Medical Science, Pla

Institute of Biotechnology, Academy of Military Medical Sciences, Pla.

Jinlong Zhang

Wei Chen

Lihua Hou

Ling Fu

Shipo Wu

Xiaohong Song

Zhe Zhang

Busen Wang

Qiang Guo

Renjie Chen

Yanbo Wen

Zhiming He

Zhenghao Zhao

Xueyun Xie

A single plasmid vector system for packaging recombinant human adenovirus type 4. The vector system contains an E3 region-deleted human adenovirus type 4 (HAdV-4 or Ad4) genome, a vector sequence for amplifying plasmids in bacteria, a pBR322 replication origin, a kanamycin resistance gene, and a replication control sequence; and an exogenous gene embedding site is located behind a packaging signal of the human adenovirus type 4 and in front of an E1 region. The present invention further provides a method for packaging the recombinant human adenovirus type 4 by the single plasmid vector system and an application in vaccine and drug preparation. The vector system can be used for rapidly and efficiently preparing a human adenovirus type 4 vector recombinant virus for stably expressing an exogenous gene, and has a good application prospect in the fields of preparation of a diagnostic kit, a vaccine, a gene therapy kit and/or a tumor therapy drug, etc.

Single plasmid vector system for packaging recombinant human adenovirus type 4 and application thereof

A charging method, including: obtaining the number of charge-discharge cycles of a battery and an application scenario during charging; determining a target charging mode among at least two charging modes according to the number of charge-discharge cycles and the application scenario, in which different charging modes have different charging rates, and the charging modes with different charging rates have different charging damages to the battery; and charging the battery in the target charging mode.

Charging method, electronic device and storage medium

A battery charging method includes: determining whether a present charging cycle satisfies a floating charging condition when a first condition is satisfied, the first condition being that in a case where battery capacity is greater than a set capacity, a duration of continuous charging is greater than a set threshold; and charging using a set floating charging mode after determining that the present charging cycle satisfies the floating charging condition. As such, the battery capacity during the charging process is detected in real time. In a case where the battery capacity is greater than the set capacity, the duration of continuous charging is greater than the set threshold and the present charging cycle satisfies the floating charging condition, the floating charging mode is used in time, such that batteries are prevented from dangerous situations such as bulging, expansion and explosion, and the safety of battery charging is improved.

Battery charging method, apparatus, and medium

A shutdown method applicable to a terminal having a rechargeable battery, the method includes: determining a first impedance and a second impedance of the rechargeable battery, wherein the first impedance is an impedance determined based on a current temperature of the rechargeable battery, and the second impedance is an impedance determined based on a current number of charge times of the rechargeable battery; determining a target impedance as a larger impedance value from the first impedance and the second impedance; determining a shutdown voltage of the terminal based on a preset open circuit voltage of the rechargeable battery, the target impedance and a current operating current of a charging circuit; and controlling the terminal to shut down, when an operating voltage of the rechargeable battery is decreased to the shutdown voltage.

Shutdown method and terminal

The present invention relates to a nucleotide sequence as shown in SEQ ID NO: 1 for encoding a Marburg virus envelope glycoprotein, and to a human replication-deficient recombinant adenovirus capable of expressing the nucleotide sequence and a preparation method therefor, as well as an application thereof in the preparation of a vaccine against Marburg virus disease. The vaccine uses an E1 and E3 deleted replication-deficient human type-5 adenovirus as a vector, and HEK293 cells integrating an adenovirus E1 gene as a packaging cell line, and a protective antigen gene carried is a codon-optimized Marburg virus Angola strain envelope glycoprotein gene. After codon optimization of the envelope glycoprotein gene, significant expression of envelope glycoprotein can be detected in transfected cells.

Recombinant human replication-deficient adenovirus comprising a modified nucleic acid encoding the Marburg virus envelope glycoprotein

Provided are an Ebola virus envelope glycoprotein (that is GP protein) codon optimized nucleotide sequence, a human replication deficient recombinant adenovirus capable of expressing the nucleotide sequence, and applications in preparing a vaccine for preventing Ebola virus diseases. The nucleotide sequence takes a replication deficient 5 type adenovirus that is lack of E1 and E3 in a combined mode as a vector. HEK293 cells that integrate adenovirus E1 genes serve as a packaging cell line, and carried protective antigenic genes are codon optimized Zaire type Ebola virus Makona strain envelope glycoprotein genes. After the envelope glycoprotein genes are optimized by codon, the expression level in transfection cells is obviously improved.

Ebola virus disease vaccine taking human replication deficient adenovirus as vector

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Beijing, China

Jinlong Zhang