Urit Medical Electronic Co., Ltd.

Jinlan Xu

Tom Cheng Xu

Kuikui Shen

Shilei Tang

Jusong Liang

Sally Li Shen

Lili Sun

Jun Liu

Chengfeng Jiang

Disclosed herein are methods, reagent kits, and compositions for performing a bisulfite conversion of DNA directly from a biological sample including a patient's urine sample or a slide-mounted FFPE tissue sample. For example, a method of performing a bisulfite conversion of DNA can comprise certain preliminary steps for processing the biological sample and transferring a portion of the processed sample into a reaction vessel containing a bisulfite mixture. The method can further comprise heating the reaction vessel containing the biological sample and the bisulfite mixture at several heating temperatures and subsequently holding the reaction vessel at a holding temperature for a holding period. The method can also comprise certain bisulfite removal steps, desulfonation steps, and removal of the desulfonation solution. A final elution step can yield the converted DNA for further downstream sequencing and analysis.

Methods and reagent kits for bisulfite conversion of DNA

Disclosed are methods, compositions, and diagnostic kits for the rapid detection of certain types of &#x3b1;-thalassemia and &#x3b2;-thalassemia. In some embodiments, a diagnostic kit, reagents, and methods are disclosed for the rapid detection of various &#x3b1;-thalassemia and &#x3b2;-thalassemia genotypes in multiple patient samples using real-time PCR. More specifically, in certain embodiments, diagnostic kits, reagents, and methods are disclosed for the rapid detection of up to seven different &#x3b1;-thalassemia genotypes and twenty different &#x3b2;-thalassemia genotypes in one single multiplex real-time PCR reaction.

Methods, compositions, and diagnostic kits for the detection of alpha and beta thalassemia

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Guilin, China

Jinlan Xu