Other

University of Virginia Patent Foundation

Jingyi Li

James P Landers

Brian Root

Daniel G Mills

Christopher Birch

Orion N Scott

David Saul

An-Chi Tsuei

Steward Dodman

Tom Moran

Stuart Knowles

Daniel A Nelson

Fernando Dias

Rick Gardner

Betina De Gorordo

Matthew Yeung

David Vigil

Jacquelyn A DuVall

Andrew Guy

Delphine Le Roux

Brandon L Thompson

Stan Wada

A collection device for a biological sample to capture target compounds such as viruses or other pathogens or particles for testing from within the sample and move the captured target compound to a separate chamber for subsequent processing. The collection device can include an openable substance blister including capture particles located in a cup interior. Capture particles can attract and bind the target compounds from the sample. An extraction tube extracts any nucleic acid from the target compound for storage or subsequent amplification and testing to confirm presence of known microorganisms. The extraction tube can comprise a heat-deformable material and can be connected to a microfluidic cartridge for further processing of nucleic acid including, amplification and detection. The microfluidic cartridge includes valves and a plurality of chambers for amplification.

Method and system for sample collection, storage, preparation and detection

A method to extract, amplify and separate nucleic acid in a microfluidic device having a plurality of chambers and channels can include a) introducing cells having nucleic acid to a first chamber of the microfluidic device and subjecting the cells in the first chamber to conditions that lyse the cells. The method can further include b) subjecting the first chamber to centrifugal force, thereby allowing the lysate or a portion thereof having nucleic acid to be distributed to a second chamber through a first channel in the microfluidic device. The method can also include c) combining the lysate or the portion thereof and reagents for amplification of the nucleic acid, thereby providing a second mixture. The method can also include d) subjecting the second chamber to centrifugal force, thereby allowing gas to be expelled from the second mixture.

Devices and methods for extraction, separation and thermocycling

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Charlottesville, VA, United States of America

Jingyi Li