International Business Machines Corporation

Illumina Corporation

Globalfoundries Inc.

Illumina Cambridge Limited

University of California

Globalfoundries U.S. 2 LLC

Jingwei Bai

Joshua T Smith

Kevin L Gunderson

Boyan Boyanov

Yann A Astier

Satyavolu S Papa Rao

Jeffrey G Mandell

Stanislav Polonsky

Kathleen B Reuter

Gustavo Alejandro Stolovitzky

Jonathan Mark Boutell

Roberto Rigatti

Matthew William Kellinger

Cheng-Yao Chen

Chao Wang

Maria Candelaria Rogert Bacigalupo

John M Beierle

Klaus Maisinger

Liangliang Qiang

Bradley Baas

Michael A Guillorn

Evan G Colgan

Qinghuang Lin

Christopher Vincent Jahnes

Robert L Bruce

Aaron Daniel Franklin

Young Hoon Kwark

Deqiang Wang

Michel Perbost

Yann A N Astier

Yann Andre Nicolas Astier

Laxmi Priya Parida

Xiangfeng Duan

Michael F Lofaro

Yu Huang

Sergio Peisajovich

Philip G Collins

Gregory A Weiss

Niina S Haiminen

The present disclosure relates to the field of molecular biology and more specifically to microarrays and methods. In particular, the present disclosure relates to a method for amplifying a nucleic acid on a substrate comprising at least one well, the method includes: depositing a plurality of first capture primer pairs and a plurality of second capture primer pairs in a layer at least partially covering the inner well surface, wherein the primer density of first capture primer pairs is higher than the primer density of second primer pairs; contacting a sample comprising a plurality of target polynucleotides with the substrate under conditions sufficient for a single target polynucleotide per well to hybridize with a capture primer of the second capture primer pair; and performing a kinetic exclusion assay (KEA) to produce a monoclonal population of amplicons from the single target polynucleotide.

Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid

The disclosure provides detection apparatus having one or more nanopores, methods for making apparatus having one or more nanopore and methods for using apparatus having one or more nanopores. Uses include, but are not limited to detection and sequencing of nucleic acids.

Hybrid nanopore sensors

The present disclosure relates to the field of molecular biology and more specifically to microarrays and methods, including methods for modifying immobilized capture primers comprising: a) contacting a substrate comprising a plurality of immobilized capture primers with a plurality of template nucleic acids under conditions sufficient for hybridization to produce one or more immobilized template nucleic acids, and b) extending one or more immobilized capture primers to produce one or more immobilized extension products complementary to the one or more template nucleic acid.

An apparatus for an electro-fluidic flow probe includes a body portion including an electro-fluidic bias tee for receiving (i) a fluid electrolyte and (ii) an electrical connection for providing an electrical potential to the fluid electrolyte; a first inlet including a tube extending from the first inlet to an outlet through the electro-fluidic bias tee; and a second inlet including the electrical connection having a wire that extends from the second inlet to the outlet through the electro-fluidic bias tee to transfer the electrical potential to a device under test.

Electro-fluidic flow probe

Provided herein are methods and compositions for placing single target molecules on a patterned substrate.

Compositions and methods for single molecular placement on a substrate

The present disclosure provides a method for sequencing nucleic acids. The method can include polymerase catalyzed incorporation of nucleotides into a nascent nucleic acid strand against a nucleic acid template, wherein the polymerase is attached to a charge sensor that detects nucleotide incorporation events. One or more non-natural nucleotide types that each produce a unique signatures at the charge sensor can be used to uniquely identify different nucleotides in the template nucleic acid.

Field-effect apparatus and methods for sequencing nucleic acids

Embodiments of the present disclosure relate to methods for capturing and amplifying target polynucleotides on a solid surface, in particular in a well in a microarray, wherein the microarray may comprise a) a substrate comprising at least one well, a surface surrounding the well and an inner well surface; b) a first layer covering the inner well surface and comprising at least one first capture primer pair; and c) a second layer covering the first layer and the surface surrounding the well.

A method of nucleic acid sequencing. The method can include the steps of (a) providing a polymerase tethered to a solid support charge sensor; (b) providing one or more nucleotides, whereby the presence of the nucleotide can be detected by the charge sensor; and (c) detecting incorporation of the nucleotide into a nascent strand complementary to a template nucleic acid.

Biochemically activated electronic device

A technique includes forming a gradient channel with width and depth gradients. A mask is disposed on top of a substrate. The mask is patterned with at least one elongated channel pattern having different elongated channel pattern widths. A channel is etched in the substrate in a single etching step, the channel having a width gradient and a corresponding depth gradient both simultaneously etched in the single etching step according to the different elongated channel pattern widths in the mask.

Nanochannel device with three dimensional gradient by single step etching for molecular detection

A nanodevice includes a nanochannel disposed through a dielectric material. A first electrode is disposed on a first side of the nanochannel, is formed within the dielectric material and has a surface exposed within the nanochannel. A second electrode is disposed on a second side of the nanochannel, is formed within the dielectric material and has a surface exposed within the nanochannel opposite the first electrode. A power circuit is connected between the first and second electrodes to create a potential difference between the first and second electrodes such that portions of a molecule can be identified by a change in electrical properties across the first and second electrodes as the molecule passes.

Tunneling junction to distinguish targeted DNA segment

Growing community of inventors

San Diego, CA, United States of America

Jingwei Bai