New England Biolabs, Inc.

Cell Signaling Technology, Inc.

Jing Zhou

Shuang-Yong Xu

Ailan Guo

Zhenyu Zhu

Michael Comb

John Edward Rush, Ii

Jing Li

Jun-Ming Cai

Adam Hume

Jeffrey Cruz Silva

Robert E Maunus

John J Pelletier

Andrew Dore

Hongbo Gu

Daniel Mulhern

There is provided a motif-specific, context-independent antibody that specifically binds a recurring, modified motif consisting of (i) at least one sumoylated lysine residue, and (ii) one or more degenerate amino acids bound by a peptide bond to said sumoylated lysine residue, said antibody specifically binding said motif in a plurality of non-homologous peptides or proteins within an organism in which it recurs. Also provided is a motif-specific, context-independent antibody that specifically binds a recurring, modified motif consisting of (i) a C-terminal aspartic acid residue, and (ii) one or more degenerate amino acids bound by a peptide bond to said C-terminal aspartic acid residue, said antibody specifically binding said motif in a plurality of non-homologous peptides or proteins within an organism in which it recurs.

Motif-specific and context-independent antibodies that specifically bind to a sumoylated lysine-containing residue

The disclosure features over 5000 methylation and acetylation sites identified in human cell line, human serum and mouse tissues, peptides (including AQUA peptides) comprising a methylation or acetylation site of the disclosure, antibodies specifically bind to a methylation or acetylation site of the disclosure, and diagnostic and therapeutic uses of the above.

Methylation and acetylation sites

Motif-specific and context-independent antibodies that specifically bind to a cleaved caspase motif

The present invention relates to recombinant DNA that encodes the BsmBI restriction endonuclease as well as BsmBI methyltransferase, expression of BsmBI restriction endonuclease and BsmBI methylase in cells containing the recombinant DNA. It also relates to a method for purification of the recombinant BsmBI restriction endonuclease.

Method of cloning and expression of BsmBI restriction endonuclease and BsmBI methylase in E. coli and purification of BsmBI endonuclease

The present invention relates to recombinant DNA which encodes the BsmAI restriction endonuclease as well as BsmAI methylase, expression of BsmAI restriction endonuclease and BsmAI methylase in cells containing the recombinant DNA, and purification of BsmAI endonuclease to near homogeneity.

Method for cloning and expression of BsmAi restriction endonuclease and BsmAI methylase in E. coli

The present invention relates to recombinant DNA encoding the BsaWI restriction endonuclease as well as BsaWI methylase, expression of BsaWI restriction endonuclease and BsaWI methylase in cells containing the recombinant DNA.

Method for cloning and expression of BsaWI restriction endonuclease and BsaWI methylase in E. coli

The present invention relates to recombinant DNA which encodes the BsmI restriction endonuclease as well as BsmI methyltransferases, expression of BsmI restriction endonuclease in cells containing the recombinant DNA by using a low copy number T7 expression vector pACYC-T7ter, and purification of BsmI restriction endonuclease by heat treatment and chromatography through heparin Sepharose column.

Method for cloning and producing the BsmI restriction endonuclease in E. coli

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Beverly, MA, United States of America

Jing Zhou