Fluidigm Corporation

Jing Wang

Andrew Paul May

David S Cohen

Robert C Jones

Marc Alexander Unger

Brian Fowler

Jason A A West

Jake Kimball

Dominique Toppani

Myo Thu Maung

Michael C Norris

Hany Ramaz Nassef

Hou-Pu Chou

Tim Woudenberg

Timothy M Woudenberg

Peilin Chen

Antoine Daridon

Paul Wyatt

Dominique G Toppani

The present invention provides reagents, kits, and methods for single-cell whole genome amplification using Phi 29 DNA polymerase.

Reagents and kit compositions for single-cell whole genome amplification

Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

Methods for multiple single-cell capturing and processing using micro fluidics

Methods, systems and devices for multiple single-cell capturing and processing using microfluidics

New high density microfluidic devices and methods provide precise metering of fluid volumes and efficient mixing of the metered volumes. A first solution is introduced into a segment of a flow channel in fluidic communication with a reaction chamber. A second solution is flowed through the segment so that the first solution is displaced into the reaction chamber, and a volume of the second solution enters the chamber. The chamber can then be isolated and reactions within the chamber can be initiated and/or detected. High throughput methods of genetic analysis can be carried out with greater accuracy than previously available.

High efficiency and high precision microfluidic devices and methods

A method for rendering a microfluidic device suitable for reuse for nucleic acid analysis is provided. The method may include flowing a nucleic acid inactivating solution into a microfluidic channel of the device by pumping; and then flowing a wash solution into the channel by pumping, thereby displacing the nucleic acid inactivating solution from the channel, whereby any residual nucleic acid from a prior use of the device is inactivated.

Microfluidic device regeneration

Microfluidic devices are described that include a rigid base layer, and an elastomeric layer on the base layer. The elastomeric layer may include at least part of a fluid channel for transporting a liquid reagent, and a vent channel that accepts gas diffusing through the elastomeric layer from the flow channel and vents it out of the elastomeric layer. The devices may also include a mixing chamber fluidly connected to the fluid channel, and a control channel overlapping with a deflectable membrane that defines a portion of the flow channel, where the control channel may be operable to change a rate at which the liquid reagent flows through the fluid channel. The devices may further include a rigid plastic layer on the elastomeric layer.

Microfluidic mixing and reaction systems for high efficiency screening

A microfluidic device is described that has a slug mixing arrangement. A reaction well has an input flow channel with a first valve near the reaction well, and a second valve further from the reaction well. A fluid source is connected to the segment in between the two valves. A second fluid source is connected behind the second valve. The channel between the two valves receives the first fluid by blind filling when the two valves are closed. The reaction well receives the first fluid followed by the second fluid when the first and second valves are open.

Microfluidic reaction apparatus for high throughput screening

An SBS-formatted microfluidic device where the geometry of the plate defines an array of interrogation areas, and where each interrogation area encompasses at least one reaction site.

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South San Francisco, CA, United States of America

Jing Wang