Massachusetts Institute of Technology

Genzyme Corporation

Jin Yin

Konstantin Konstantinov

Lev E Bromberg

Veena Warikoo

T Alan Hatton

Bernat Olle

Kevin Brower

Daniel I C Wang

Rao Koduri

Marcella Yu

Christopher Hwang

Hang Zhou

Benjamin Wright

Chris Hwang

Kevin P Brower

Disclosed herein are methods that have been developed to control the formation of disulfide bonds between polypeptides of a multimeric protein produced by a bioprocess. Also disclosed are protein solution parameters that allow for controlling the formation of disulfide bonds. In one example, the methods disclosed herein can be used to control the proportion of half antibody molecules in an antibody solution.

Methods of controlling the formation of disulfide bonds in protein solutions

One aspect of the present invention relates to magnetic nanoparticles colloidally stabilized in aqueous milieu by association with an organic phase. The organic phase may be either a fluorinated polymer or an organic hydrocarbon bilayer, wherein the two layers are chemically bonded to each other. The stabilized particles are further non-toxic and provide useful enhancements in bioprocesses. Another aspect of the present invention relates to compositions comprising an oxygen-dissolving fluid vehicle and surface modified, nanometer-sized magnetic particles. The inventive compositions have utility in a wide range of applications, but are particularly suitable for use as recyclable oxygen carriers, separation and purification vehicles, and bioprocessing media, including fermentation processes.

Bioprocesses enhanced by magnetic nanoparticles

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Somerville, MA, United States of America

Jin Yin