Shenzhen Bluepha Biosciences Co., Ltd.

Jin Yin

Yu Wang

Jie Hou

Liang Zou

Pingan Tang

Huayu Zhang

Xiaocui Xie

Tian Liu

Jiajia Li

Zixian Chen

Yakun Wu

Yingzi Li

The present invention relates to the technical field of microorganisms, and specifically to engineered microorganisms expressing acetoacetyl-CoA reductase variants and methods for increasing the proportion of 3-hydroxyhexanoic acid in PHA. The acetoacetyl-CoA reductase variants and their coding genes provided by the present invention can significantly increase the proportion of 3-hydroxyhexanoic acid in PHA produced by strains; the proportion of 3-hydroxyhexanoic acid in PHA produced by the engineeredconstructed utilizing the acetoacetyl-CoA reductase variants and their coding genes provided by the present invention is significantly increased, which provides new genes and strain resources for strains producing poly(3-hydroxybutyrate-co-3-10 the development of engineered hydroxyhexanoate) with high proportion of 3-hydroxyhexanoic acid.

Engineered microorganisms expressing acetoacetyl-COA reductase variants and methods for increasing proportion of 3-hydroxyhexanoic acid in PHA

A recombinant bacterium with a high PHA yield and its construction method provide an engineered microorganism that can be used for the production of polyhydroxyalkanoate (PHA). A recombinantwith a high PHA yield includes a promoter for upregulating a phaJ gene and further includes a mutant phaC gene. The recombinantcan be used for producing 3-hydroxybutyrate-co-3-hydroxyhexanoate (PHBHHx) in PHA.

Recombinant bacterium with a high PHA yield and the construction method thereof

Provided is engineered microorganisms expressing acetoacetyl-CoA reductase variants and a method for improving the yield of PHA. Compared with the wild-type acetoacetyl-CoA reductase represented by SEQ ID NO. 31, the variant has one or more of the following mutations: (1) mutation of valine at position 141 to isoleucine or leucine; (2) mutation of methionine at position 12 to threonine, serine, alanine, leucine, lysine or isoleucine; (3) mutation of isoleucine at position 194 to valine, leucine or methionine; (4) mutation of glutamic acid at position 42 to lysine, glutamine, leucine, aspartic acid, proline, threonine, asparagine, or histidine; and (5) mutation of phenylalanine at position 55 to valine, alanine or isoleucine. The variants and their coding genes can promote the synthesis and accumulation of PHA by the strain and increase the yield of PHA.

Engineered microorganisms expressing acetoacetyl-CoA reductase variants and method for improving the yield of PHA

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Guangdong, China

Jin Yin