Development Center for Biotechnology

Jin-Tann Chen

Hsin Tsai

Hsiu-Hui Chen

Same-Ting Chen

Disclosed is a mutant aspartase comprising the amino acid sequence of a wild-type E. coli aspartase wherein the amino acid residue at one of positions 25, 123, 421 and 463 is substituted. Also disclosed is a process for preparing the above mutant aspartase.

Mutant aspartase and the preparation thereof

A process for the preparation of monohydrated sodium phenylpyruvate comprises reacting benzaldehyde with the stoichiometric quantity of hydantoin in the aqueous medium in the presence of a catalytic quantity of a primary or secondary amine at high temperature, treating the reaction mixture with an excess of sodium hydroxide at high temperature, adding sodium chloride to the sodium hydroxide-treated solution, acidifying the so obtained solution with concentrated hydrochloric acid, and washing the formed precipitate with methanol.

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Taipei, Taiwan

Jin-Tann Chen